摘要
目的探讨热疗对膀胱癌细胞耐药性的影响。方法不同温度条件下热疗与化疗联合作用耐药膀胱癌细胞株BIU-87后,用MTT法检测细胞增殖,流式细胞仪检测细胞周期、细胞凋亡和survivin表达的变化。结果热疗可显著增强羟基喜树碱对BIU-87的抑制作用,并可减少survivin的表达。热疗可以诱导BIU-87的凋亡,并呈温度依赖性。结论热疗可增强化疗药物作用,部分逆转膀胱癌细胞的耐药性,并促进凋亡。
Objective To investigate the effect of hyperthermia on multidrug resistance in BIU-87 cell line.Methods BIU-87 cells were treated by hydroxycamptothecin at different temperature （37℃43℃）,and then subjected to the proliferation assay by MTT.The expression of survivin,cell cycle and apoptosis were determined by flow cytometry.Results Hyperthermia enhanced the inhibitory effects of hydroxycamptothecin on BIU-87 cells and reduced the expression of survivin.Hyperthemia could induce the apoptosis of BIU-87 cell in temperature-dependant manner.Conclusion Hyperthermia can enhance the inhibition rate of chemotherapeutics drugs,partially reverse the multidrug resistance and induce the apoptosis of BIU-87 cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2011年第1期23-26,共4页
Journal of China Medical University
基金
辽宁省博士启动基金资助项目(20101139)
关键词
膀胱移行细胞癌
BIU-87细胞株
热疗
凋亡
生存素
bladder transitional cell carcinoma
BIU-87 cell line
hyperthemfia
apoptosis
survivin
