摘要
本文介绍利用圆二色(CD)光谱法研究20 keV N+注入对α-淀粉酶二级结构的影响,测定其CD光谱,分析了酶活性变化的原因。结果表明:在1015–1016/cm2范围,不同注量的低能N+注入对α-淀粉酶的α-螺旋、β-折叠、β-转角及无规卷曲相对含量的影响程度不同。低能N+注入使α-淀粉酶活性发生改变,酶活性的改变与其空间结构的变化密不可分。
Samples of the α-amylase were treated with 10^15-10^16 ions/cm2 of 20 keV N＋. The secondary structure of the ion-implanted α-amylase was studied by circular dichroism, and activity change of the α-amylase was analyzed. The results showed that in the dose range under investigation, the N＋ implantation affected the relative contents of α-helix, β-sheet, β-turn and random coil of the α-amylase, but no significant differences were found between the samples implanted to different doses. The activity of the N＋-implanted α-amylase changed, with a relationship between the activity increase and the conformation change.
出处
《核技术》
CAS
CSCD
北大核心
2011年第2期147-150,共4页
Nuclear Techniques
基金
陕西省科学技术研究计划(2010K01-078)项目
陕西省教育厅自然科学专项基金(08JK214)
宝鸡文理学院重点项目(ZK1015)
关键词
离子注入
Α-淀粉酶
圆二色光谱
二级结构
酶活性
Ion beam implantation, α-amylase, Circular dichroism, Secondary structure, Enzyme activity
