摘要
目的 比较ＤＣ对诱导４ ｄ（Ｌ４） 和诱导７ ｄ（Ｌ７） 的ＬＰＡＫ 细胞体外杀伤人肝癌细胞株ＢＥＬ－ ７４０２（Ｂ） 的杀伤力和杀伤模式的影响．方法 Ｌ４ 组为Ｂ＋ Ｌ４ ，ＬＤ４ 组为Ｌ４ 组＋ ＤＣ；Ｌ７ 组为Ｂ＋ Ｌ７ ，ＬＤ７ 组为Ｌ７ 组＋ ＤＣ．Ｌ４ 和Ｌ７ 与Ｂ的效靶比均为５∶１ 和１０∶１两种． 采用杀伤细胞检测技术及电镜技术，比较各组的杀伤效应和杀伤模式．结果 各组的细胞毒活性为ＬＤ４ ＞ Ｌ４（ Ｐ＜ ０－０１） ，ＬＤ７ ＞ Ｌ７（ Ｐ＜ ０－０１） ．Ｌ４ 组和ＬＤ４ 组的ＢＥＬ７４０２ 细胞呈现不同程度的变性坏死，Ｌ７ 组和ＬＤ７ 组的ＢＥＬ７４０２ 细胞则呈现不同程度的凋亡改变．结论 ＤＣ对不同诱导时间的ＬＰＡＫ 细胞体外杀伤肿瘤细胞都有明显的促进作用，但并不改变ＬＰＡＫ 细胞对肿瘤细胞的杀伤模式．
AIM To study the efficiency of dendritic cells (DC) on LPAK cells induced for 4 days (L 4) and 7 days (L 7) in killing human hepatoma cell line BEL 7402(B) in vitro, including killing activity and model. METHODS Two kinds of ratios of effect and target (5∶1 and 10∶1) were used in L4 group (B+L 4), LD4 group (B+L 4+DC), L7 group (B+L 7) and LD7 group (B+L-7+DC). The techniques of cell killing detection and electronic microscopy were adopted. RESULTS The cytotoxic activities were LD4>L4 ( P <0 01),LD7>L7 ( P <0 01). The BEL 7402 cells in L4 and LD4 groups revealed denaturation and necrosis in each degree, but the BEL 7402 cells in L7 and LD7 groups manifested apoptotic change in a certain degree. CONCLUSION DC could enhance the killing activity of LPAK cells induced at different times against tumor cells in vitro, but they could not change the killing model of LPAK cells.
出处
《世界华人消化杂志》
CAS
1999年第8期673-675,共3页
World Chinese Journal of Digestology
基金
广东省高教厅自然科学重点资助项目
