摘要
目的 研究与致癌物亚硝胺代谢激活有关的细胞色素Ｐ４５０２ Ｅ１ （ ＣＹＰ２ Ｅ１） 和与致癌物代谢解毒有关的谷胱甘肽转硫酶（ ＧＳＴＭ１） 基因多态与胃癌易患性，探讨环境基因在胃癌发病危险中的作用．方法 应用分子流行病学方法，在胃癌高发区福建省长乐市配比调查１０１ 对原发性胃癌病例和按性别、年龄、民族配对的健康对照，ＰＣＲ－ ＲＦＬＰ 方法检测 ＣＹＰ２Ｅ１ 和 ＧＳＴＭ１ 基因型，条件Ｌｏｇｉｓｔｉｃ 回归模型进行资料分析．结果 胃癌病例平均年龄５８－９ 岁±１１－０ 岁，对照平均年龄５８－６ 岁±１１－１ 岁，两组年龄性别均衡可比． 胃癌病例ＣＹＰ２Ｅ１的Ｃ１／ Ｃ２ 或Ｃ２／ Ｃ２ 基因频率高于对照，但差异无显著性意义〔比值比（ ＯＲ） ＝ ３－１０ ，９５ ％ 可信限（ ＣＩ） ＝ ０－９７ － ９－９１ 〕．ＧＳＴＭ１ 基因缺失与胃癌易患性有关（ ＯＲ ＝ ３－２７ ，９５ ％ ＣＩ＝１－１４ ～９－３９） ． 胃癌高发区居民长期食用鱼露（ ＯＲ ＝ １４－７４ ，９５ ％ ＣＩ＝ ２－９６ ～７３－４３） 、少吃新鲜蔬菜（ ＯＲ＝ １１－３４ ，９５ ％ ＣＩ＝１－９８ ～６４－７８） 和不良饮食习惯（ ＯＲ ＝ ２－５５ ，９５ ％ ＣＩ＝ １－１４ ～５－?
AIM To study the association between genetic polymorphisms of cytochrome P 4502E1 ( CYP 2E1) and/?or glutathione S-transfarase M1 ( GS TM1) and susceptibility to gastric cancer, and to investigate the interaction of genetic and environmental factors in etiology of gastric cancer. METHODS A 1∶1 matched case-control study was conducted in Changle, Fujian Province, a high gastric cancer mortality area. A total of 101 pairs of cases and controls were matched on sex, age and nationality. Genotypes of the CYP 2E1 and GST M1 genes were determined by the polymerase chain reaction. The methods of analysis was conditional logistic regression analysis. RESULTS Gastric cases were more likely to carry genotypes C1/?C2 and C2/?C2 than controls, 〔odds ratio (OR)=3 10,95% confidence interval (CI)=0 97-9 91〕. GSTM1 null genotype was a genetic susceptibility factor (OR=3 27,95%CI=1 14-9 39). Fish sauce intake(OR=14 74,95%CI=2 96-73 43),less consumption of fresh vegetables (OR=11 34,95%CI=1 98-64 78),and bad eating habits (OR=2 55,95%CI=1 14-5 73)were the risk factors of gastric cancer. CONCLUSION Genetic polymorphisms in enzymes involved in carcinogen metabolism and environmental risk factors may play an important role in the development of gastric cancer.
出处
《世界华人消化杂志》
CAS
1999年第8期652-655,共4页
World Chinese Journal of Digestology
基金
福建省科委基金
关键词
胃肿瘤
遗传多态性
细胞色素P450
危险因素
stomach neoplasms
genetic polymorphisms
cytochrome P450
risk factors
glutathione S-transferase
case control studies
