摘要
优化了苯酚-硫酸法测定仙人掌多糖含量的条件:取多糖样品2 m1,加4%苯酚0.5 m1,迅速加入浓硫酸5 ml,摇匀后静置5 min,于482 nm处测定吸光度。多糖在7.5～37.5μg/ml浓度范围内与吸光度的线性关系良好。采用果胶酶和纤维素酶双酶提取法从仙人掌中提取仙人掌多糖,最佳提取条件为:果胶酶浓度0.7%,纤维素酶浓度0.3%,40℃提取1.5 h。仙人掌多糖产率为20.08%,纯度为83.02%。
The conditions for the determination of polysaccharides from the wild Opuntia dillenii Haw.with phenol-sulfuric acid method were optimized,which were as follows：2 ml of Opuntia dillenii Haw.samples,0.5 ml of 4%phenol and 5 ml of sulphuric acid,shaken up and placed stationarily for 5 min.The samples were measured by spectrophotometer at the wavelength of 482 nm.The calibration curve was linear in the concentration range of 7.5 - 37.5μg/ml.The extraction of polysaccharides from Opuntia dillenii Haw.was performed after sample was treated by 0.7%pectinase and 0.3%cellulase at 40℃for 1.5 h.Under these conditions,the yield of polysaccharides was 20.08% with purity of 83.02%.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2011年第2期96-100,共5页
Chinese Journal of Pharmaceuticals
基金
广东省自然科学基金(8152404801000011)
广东省教育厅基金(Z03062)
关键词
仙人掌
多糖
苯酚-硫酸法
果胶酶
纤维素酶
提取
优化
Opuntia dillenii Haw.
polysaccharides
phenol-sulfuric acid method
pectinase
cellulase
extraction
optimization
