摘要
采用毛细管区带电泳(CZE)对随机尿中香草扁桃酸(Vanillylmandelic Acid,VMA)、肌酐(Creatinine,CR)进行测定。实验优化了缓冲介质、分离电压、毛细管内径等参数。结果表明:在优化条件下,VMA和CR在13 min内可以实现分离检测,VMA和CR分别在0～500μmol/L和0～4 000μmol/L范围内与峰面积呈良好的线性关系,检出限(S/N=3)分别为1.0μmol/L和50.0μmol/L。VMA和CR迁移时间和峰面积的相对标准偏差(RSD)分别为0.57%、0.45%和3.78%、2.78%,回收率分别为98.36%和98.85%。高香草酸(HVA)、儿茶酚胺、5-羟色胺和清蛋白等对测定结果无干扰。该法用于尿液中VMA和CR检测,取得了满意的结果。
Determination of vanillylmandelic（VMA） and Creatinine（CR） in random urine was performed by capillary zone electrophoresis.The effets of some important factors including the running buffer,the separation voltage and the inner diameter of capillary on the separation were investigated.Under the optimum conditions,vanillylmandelic and Creatinine was separated and detected in 13 min.The linear ranges of VMA and CR were 0～500 and 0～4 000 μmol/L,and the detection limits were 1.0 μmol/L for VMA and 50.0 μmol/L for CR.The relative standard deviations（n=10） of migration time and peak atea of VMA and CR were 0.57%、0.45% and 3.78%、2.78%,respectively.No obvious interference caused by other compounds in human urine such as catecholamines,5-hydroxytryptamine and albumen was observed.The method was applied to the determination of VMA and CR in random urine with satisfactory results.
出处
《分析科学学报》
CAS
CSCD
北大核心
2011年第1期77-80,共4页
Journal of Analytical Science
基金
连云港市社会发展基金(No.06056)
关键词
高效毛细管电泳
香草扁桃酸
肌酐
Capillary electrophoresis
Vanillylmandelic
Creatinine
