摘要
制备了纳米颗粒的银溶胶,研究了核黄素水溶液中加入纳米银后荧光光谱和吸收光谱的变化。纳米银可以使核黄素的荧光发生猝灭,它们之间的相互作用属于静态猝灭机制,求出了猝灭常数Ksv=1.04×104L/mol,Kq=1.5×1013L.mol-1.s-1。纳米银对核黄素荧光的这种静态猝灭引起吸收光谱发生变化,随着纳米银浓度的增大,吸收强度也不断增大,而且吸收峰从371 nm红移至376 nm、445 nm蓝移至441 nm。研究表明,核黄素结构中发色团上尿嘧啶端的氮杂原子及羰基中的氧原子与纳米银形成了复合物,核黄素与纳米银之间存在能量转移,从而导致了核黄素光谱的变化。
The influence of nanosilver on optical property of riboflavin solution has been studied by measuring their absorption and fluorescence spectra.The fluorescent emission intensity of riboflavin solution was quenched by nanosilver.The dominant process for this quenching might be attributed to static quenching.The static quenching constant Ksv and quenching rate constant Kq were identified at 1.04×104 L/mol and 1.5×1013 L·mol-1·s-1,respectively,according to the Stern-Volmer equation.The absorption peak position of the riboflavin solution was influenced by the nanosilver.The absorption peak of riboflavin was shifted,from 371 nm to 376 nm and from 445 nm to 441 nm,respectively,when nanosilver was added.The absorption intensity increased with an increasing nanosilver concentration.The test results suggested that the nitrogen atoms of the uracil end and oxygen atoms in carbonyl of riboflavin structure formed metal complexes on the surface of nanosilver.The energy was transferred from riboflavin to nanosilver,resulting in a change of riboflavin spectra.
出处
《化学试剂》
CAS
CSCD
北大核心
2011年第2期149-151,163,共4页
Chemical Reagents
基金
国家自然科学基金资助项目(60671010
2077-5045)
滨州医学院校级资助项目(BY2009KJ30)
关键词
纳米银
核黄素
荧光猝灭
吸收光谱
nanosilver
riboflavin
fluorescence quenching
absorption spectra
