摘要
目的观察温郁金醚提物中二萜类化合物C(以下简称化合物C)体外对人结肠癌细胞SW 620生长、细胞凋亡和细胞周期的影响。方法以顺铂(DDP)为阳性对照药物,采用噻唑蓝(MTT)法检测化合物C对SW620细胞增殖的抑制作用;An-nexin V-FITC标记流式细胞术(FCM)检测化合物C对SW620细胞的凋亡抑制率,流式细胞术检测化合物C对SW 620细胞周期的影响。结果 MTT法显示化合物C对SW620的半数抑制浓度(IC50)为24.16μg.mL-1,顺铂为45.80μg.mL-1;化合物C较低浓度时的抑制率与阳性对照组(DDP组)相似,在30,50,70μg.mL-1时的抑制率与对照组比较,差异有统计学意义(均P<0.05);FCM显示,化合物C能诱导SW 620细胞凋亡,其诱导凋亡率呈现一定的浓度和时间依赖性,48 h后化合物C70μg.mL-1组凋亡率为33.55%;化合物C能将SW 620细胞阻滞于G1期,减少肿瘤细胞在S期和G2/M的比例。结论化合物C能诱导SW 620细胞凋亡,阻滞其细胞周期,抑制细胞增殖,是温郁金发挥抗肿瘤作用的有效成分之一。
Objective To investigate the effect of diterpenoid curcumrinol C from ether extract of curcuma wenyujin（compound C） on cell growth,apoptosis and cell cycle of human colon cancer cell line SW620 in vitro. Methods The human colon carcinoma cell SW620 were cultured and treated with the compound C at different concentrations compared with cisplatin（DDP）.MTT assay was used to test dynamically the cell growth inhibiting effect of curcumrinol C.Apoptosis induction（AnnexinV-FITC/PI） and modulation of DNA cell cycle was detected by flow cytometry（FCM）. Results IC50 value of the compound C and DDP were 24.16 and 45.80 μg·mL-1,respectively.It was shown that the compound C at a low dose had similar effects to the positive control,obvious difference was developed in compound C at 30,50,70 μg·mL-1（P0.05）.The compound C induced SW620 cells apoptosis in a dose and time dependent manner,with inhibition percentage of 33.55% at the dose of 70 μg·mL-1 for 48 h by FCM.The cell cycle was arrested at phase G1 treated by compound C and the ratio of cancer cells at phase S and G2/M was dropped. Conclusion The compound C induces cell line SW620 apoptosis,blocks cell cycle and inhibits cell proliferation,which is one of the active anti-tumor components in curcuma wenyujin.
出处
《医药导报》
CAS
2011年第2期160-163,共4页
Herald of Medicine
基金
浙江省中医药管理局基金资助项目(基金编号:2009CB014)
关键词
温郁金
结肠癌
细胞增殖
凋亡
Curcuma wenyujin
Colon carcinoma
Proliferation
Apoptosis
