摘要
以连翘籽苗为材料,采用秋水仙素溶液处理生长点的诱变,茎尖细胞染色体计数检测及流式细胞仪倍性鉴定等方法,研究了不同浓度秋水仙素对连翘的诱变效果。结果表明:0.2%的秋水仙素溶液处理连翘籽苗生长点72 h变异株率最高,达到58.3%,诱导效果最佳。经秋水仙素诱导后形成的多倍体植株叶片变宽变大,叶色变深,茎变粗且节距变大,气孔增大,单位叶面积气孔数目减少,保卫细胞叶绿体数明显增多。经茎尖细胞染色体计数检测,部分变异植株茎尖细胞染色体整倍体数目为2n=56条,而二倍体的染色体数目为2n=2x=28条,因此,部分变异植株(2n=4x=56)为四倍体。经流式细胞仪倍性分析验证,连翘四倍体体细胞DNA相对含量是二倍体的2倍。检测发现有少数个体为非整倍体变异。
With Forsythia seedlings as the experimental materials,the colchicine growing point of the mutagenic solution treatment method and the number of shoot-tip cell chromosome ploidy detection method were used to study the different colchicine concentration and mutagenic effects of processing time.The results showed that 0.2% colchicine solution dealt with the highest rate of growth point mutant 72 h reached 58.3% with the best results.Compared with the original diploid plants,colchicine induced by the formation of polyploid plants in the form on the plant leaves became many times larger,whose leaf discoloration turned more intense,stems thicker,and pitch and pores larger,with the unit leaf area reducing the number of stomata,thereby significantly increasing the number of guard cell chloroplasts.Cytological studies on the variation of plants found that somatic chromosome number of the mid-2n = 56 article the original diploid chromosome number of 2n=2x=28 article,i.e.,variation plants（2n=4x=56）.Ploidy analysis by flow cytometry was thus verified： forsythia relative content of tetraploid somatic cell is twice the diploid DNA for the tetraploid.In addition,aneuploid variation was detected in a small number of individuals.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2011年第1期73-77,共5页
Journal of Hebei Agricultural University
基金
国家发改委中药材扶持项目(2006BAI06A13-03)
河北省教育厅项目(2007464)
关键词
连翘
多倍体诱导
倍性鉴定
秋水仙素
Forsythia suspendse
polyploid induction
chromosome identification
colchicine
