摘要
目的:研究微重力环境"航天贫血症"发生的相关机制。方法:采用美国国家航空航天局提供的旋转式细胞培养系统(RCCS-1)模拟微重力环境,培养人白血病K562细胞。用牛巴氏计数板计数细胞,用流式细胞术检测细胞周期,用Western-bloting方法检测细胞外信号调节激酶1/2(ERK1/2)蛋白表达及其磷酸化水平。结果:模拟微重力环境培养的K562细胞的增殖速度显著低于地面普通培养对照组;同时还显著抑制细胞周期进程,使其停滞在G0/G1期;模拟微重力环境培养的细胞磷酸化ERK1/2表达随时间延长逐渐下降。结论:模拟微重力环境使细胞周期阻滞于G0/G1期,可能是由模拟微重力作用下ERK1/2磷酸化水平下降所致。
Objective:Astronauts in space flight conditions may occur 'space anemia'. The experiment was to study the mechanisms of how 'space anemia'occurs in microgravity environment.Method:The proliferation of human leukemia K562 cells in the microgravity environment simulated by the U.S. National Aeronautics and Space Administration rotary cell culture system (RCCS-1) was studyed. We conducted cell number by a Pasteurized cow counting board,used flow cytometry to analyze the cell cycle,and Western-bloting method for detecting of phosphorylation and total protein expression of ERK1/2.Results:In simulated microgravity environment,the proliferation rate of K562 cells was significantly lower than the control group. Simulated microgravity significantly inhibited cell cycle progression,and the cell cycle was arrested in G0/G1 phase. The expression of ERK1/2 phosphorylation was decerased in simulated microgravity environment.Conclusion:The microgravity environment inhibited the proliferation of K562 cell. The cell cycle was arrested in G0/G1 phase. This may be caused by the decresed ERK1/2 phosphorylation in simulated microgravity environment.
出处
《微循环学杂志》
2011年第1期21-23,I0001,共4页
Chinese Journal of Microcirculation
基金
国防科工委专项课题分题(B3320062101)资助
