摘要
目的对绿脓杆菌的耐药性进行调查及质粒图谱分析。方法按美国临床实验室标准委员会（ＮＣＣＬＳ）推荐的标准方法（Ｋ－Ｂ法）进行药敏试验。结果绿脓杆菌对多种抗菌药物有较高的耐药率，其中氧哌嗪青霉素３９．８４％，庆大霉素６３．４１％，丁胺卡那霉素３９．８４％，头孢氨噻肟６１．７９％，头孢哌酮４２．２８％，头孢三嗪５６．１０％，多粘菌素４．０６％，头孢噻甲羧肟３５．７９％，舒普深２６．８３％，环丙沙星５０．４１％，氧氟沙星３６．５９％，左旋氧氟沙星３２．５２％，亚胺硫霉素１７．０７％，乙基西索米星６４．２２％。对其中１０株菌株进行质粒图谱分析均发现含一个２３ＫＢ的质粒。结论抗菌药物的广泛应用与细菌的高耐药性密切相关。２３ＫＢ可能导致细菌的高耐药性产生。
Objective To
investigate the resistance of Pseudomonas aeruginosa and analyzed the plasmid atlas.
Methods Antibacterial susceptivity testing was performed with disk diffusion(K-B method)
recommended by the NCCLS. Results Pseudomonas aeruginosa was resistant to most
antibacterials . In 14 drugs the resistant rates were: Piper callin 39.84%, Gentamycin 63.41%,
Amikacin 39.84%, Netilmicin 64.22%, Cefotaxine 61.79%, Cefmeroxine 35.79%, Roceph 56.10%,
Cefobide 42.28%, Sulperazon 26.83%, Cirproploxacin 50.41%, Oxo-loxacin 36.59%, L-ofloxacin
32.52%, Imipenem 17.07%, PloymyxinB 4.06%. Analyzed the plasmid in 10 straines , all of
them had a 23KB plasmid.Z Conclusion The high resistance was related to popularity of the
antibacterial used; 23KB plasimd probably leaded to the high resistance of some bacteria.
关键词
铜绿假单胞菌
耐药性
质粒
seudomonas aeruginosaDrug resistancePlasmids
