摘要
目的:检测微小RNA(miRNAs)在子痫前期患者与正常孕妇胎盘组织中的表达差异。方法:选取剖宫产分娩的产妇,其中重度子痫前期患者8例(sPE组),正常妊娠妇女8例(对照组),收集其胎盘组织。通过miRNA芯片技术检测sPE与对照组中miRNAs的表达差异。结果:与正常胎盘相比,sPE胎盘组织中存在5个表达失衡的miRNA簇(P<0.05),分别位于染色体19q13.42(miR-517*、miR-518b、miR-519e*)、13q31.3(miR-18a、miR-19a)、Xq26.2(miR-18b、miR-363)、Xq26.3(miR-542-3p、miR-450)以及人类印记基因位点14q32.31(miR-411、miR-377、miR-154*)上。结论:miRNA的表达异常可能参与了子痫前期的发生和病理生理过程。
Objective：To investigate the differential expression of miRNA in placentas from women with preeclampsia and normal pregnancy.Methods：Placentas were obtained from the patients with severe preeclampsia as sPE group（n=8） and normal pregnant women as control group（n=8）.The miRNA expression was assessed by miRNA microarray analysis.Results：5 miRNA clusters on human chromosome 19q13.42（miR-517＊,miR-518b,miR-519e＊）,13q31.3（miR-18a,miR-19a）,Xq26.2（miR-18b,miR-363）,Xq26.3（miR-542-3p,miR-450）,and a human imprinted region 14q32.31（411,miR-377,miR-154＊） were expressed differentially in preeclamptic placentas,compared with normal placenas（P0.05）.Conclusion：The differential expression of miRNA suggests the involvement of these microRNAs in the development and pathogenesis of preeclampsia.
出处
《现代医药卫生》
2011年第1期3-4,共2页
Journal of Modern Medicine & Health
基金
国家自然科学基金项目(编号:30070787)资助
