摘要
试验研究了２株白僵菌和３株绿僵菌的分生孢子在高温短时处理及高温培养条件下的萌发和成活。３５℃短时处理对孢子的萌发和生长基本无影响。４０～５０℃是孢子的敏感区。从高纬度地区吉林采集的菌株Ｂ９对高温最敏感，在４０℃处理１０分钟，发芽率下降８０％。采自非洲的菌株Ｍ１８９对高温的抵抗力最强，在４０℃处理４小时和５０℃处理１小时，孢子的发芽率保持在４０％以上，６０℃处理２０分钟仍有大约１％的发芽率。高温培养结果表明，３５℃时只有Ｍ１８９的成菌落数为６７．７％，其它４个菌株均在１％以下。
The conidia of 2 isolates of Beauveria and 3 isolates of Metarhizium were heat treated in water bath and in oven at 35￣100℃ for 10￣60 min. The germination and growth of the conidia was not influenced at 35℃ and the sensitive range of these conidia was at 40￣50℃. The isolate B9 collected from Jilin province in north China, was the most sensitive to high temperature among the 5 isolates, and only 20% of the conidia of this isolate germinated when treated at 40℃ for 10 min. The isolate M189 collected from Africa had an especially high tolerance to temperature. The rate of conidium germination still maintained above 40% at 40℃ for 4 h or at 50℃ for 1 h. About 1% of the conidia could germinate at 60℃ for 20 min. The result of the culture on PPDA(modified PDA) plates showed that the colony forming unit (CFU) of the isolate M189 was 67.7% and the other 4 isolates′ were less than 1% at 35℃.
出处
《中国生物防治》
CSCD
1999年第3期111-114,共4页
Chinese Journal of Biological Control
基金
"九五"国家攻关项目
关键词
白僵菌
绿僵菌
分生孢子
高温
耐受力
Beauveria
Metarhizium
conidia
high temperature
tolerance
