摘要
通过平板培养试验及袋栽试验,观察米糠和光皮树籽粕对平菇受铅、汞毒害的缓解效果。结果表明:当Pb2+、Hg2+的质量浓度分别达到50、15 mg/L时,对应的基础培养基中平菇菌丝的生长受到显著抑制;在正常的培养基中添加米糠和光皮树籽粕能显著促进平菇菌丝的生长(20.0 g/L最佳),同时米糠和光皮树籽粕对Pb2+、Hg2+具有螯合作用;在受到50 mg/L Pb2+或15 mg/L Hg2+污染的培养基中,添加米糠或光皮树籽粕都具有显著的螯合解毒效果,受Pb2+、Hg2+毒害的平菇菌丝可以恢复生长。袋栽试验中,在被污染的栽培料中添加米糠或光皮树籽粕,表现出很强的螯合解毒和增产效果,其平菇子实体中的Pb2+、Hg2+含量都较相应的对照栽培处理配方有显著降低。
To alleviate the toxic effects of heavy metals on Pleurotus ostreatus,detoxification of rice bran and Swida wilsoniana oilcake to heavy metals was studied through a series of plate cultivation and sawdust cultivation.The results showed that the mycelium growth of Pleurotus ostreatus were all significantly inhibited when the basic cultivating medium was contaminated by 50 mg/L Pb2+ or 15 mg/L Hg2+,respectively.Both of rice bran and Swida wilsoniana oilcake can promote the mycelium growth of Pleurotus ostreatus remarkably when they was added to the basic medium,and the best concentration was 20 g/L.Meanwhile,rice bran and Swida wilsoniana oilcake can take chelation on Pb2+ or Hg2+.The chelation capacity of rice bran on Hg2+ is one order of magnitude stronger than that of Swida wilsoniana oilcake.When rice bran and Swida wilsoniana oilcake are added in the medium contaminated by Pb2+ or Hg2+ respectively,the toxicity of Pb2+,Hg2+ to mycelium growth was decreased and the growth ofPleurotus ostreatus was recovered.In cultivation experiments,rice bran and Swida wilsoniana oilcake showed a very strong effects on chelate detoxification and increased yield as they were added in cultivation materials.Analyzing concentration of heavy metals in fruiting bodies of Pleurotus ostreatus by different cultivation methods,it was found that the concentration of Pb2+or Hg2+ were decreased when rice bran and Swida wilsoniana oilcake were added in corresponding cultivation materials,especially the detecting results of tests about lead conformed to the hygienic standard for edible fungi in China.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第1期115-120,共6页
Journal of Huazhong Agricultural University
基金
江西省自然科学基金项目(2008GZH0047)
江西省农业产业化重大项目(2007BN12100)
关键词
重金属
平菇菌丝
米糠
光皮树籽粕
解毒作用
heavy metals
Pleurotus ostreatus
rice bran
Swida wilsoniana oilcake
detoxification