摘要
在离体条件下进行患病鱼体内分离的嗜水气单胞菌3个菌株对四环素类和氟喹诺酮类药物的耐药性获得、稳定性、保存条件和交叉耐药性研究。结果表明:分别在含有盐酸多西环素、盐酸四环素、诺氟沙星和左氧氟沙星的药物培养基中连续传代9次(在28℃条件下培养72 h为1代)后,四环素类对3个菌株的最小抑菌浓度上升倍数为8～32倍,氟喹诺酮类组MIC上升倍数为125～7 997倍,且耐药获得后保持稳定。同时嗜水气单胞菌对四环素类和氟喹诺酮类药物存在交叉耐药。耐药菌4℃保存10和20 d耐药性保持稳定,30 d耐药性均有不同程度的下降。
The aims of this study were to investigate the development,stability,cross-resistance and preservation of Aeromonas hydrophila resistance to tetracyclines and fluoroquinolones. A.hydrophila was grown at 28 ℃ for 72 h and used it to test for the development of resistance after 9 sequential subcultures in sub-inhibitory concentrations of two tetracyclines（doxycycline and tetracycline） and two fluoroquinolones （norfloxacin and levofloxacin）.After 9 subcultures the minimal inhibitory concentrations （MIC） to tetracyclines were 8-32 times greater than the initial values,MIC values to fluoroquinolones were 125-7 997 times greater than the initial values,and drug-resistance were stable.The doxycycline-resistant isolate was resistant to tetracycline and fluoroquinolones,and the levofloxacin-resistant isolate was resistant to tetracyclines and norfloxacin.The drug-resistance stability test indicated that long-term storage of the bacteria at 4℃ could reduce their resistance to antimicrobials.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第1期89-93,共5页
Journal of Huazhong Agricultural University
基金
农业公益性行业科研专项(200803013)
关键词
嗜水气单胞菌
四环素类
氟喹诺酮类
耐药性获得
耐药稳定性
交叉耐药
保存
Aeromonashydrophila
tetracyclines
fluoroquinolones
drug-resistance development
drug-resistance stability
cross-resistance
preservation
