摘要
目的 探讨谷氨酰胺（Glu）对内毒素血症大鼠肠道损伤的保护作用以及对血红素加氧酶-1（HO-1）表达的影响.方法 按随机数字表法将32只雄性SD 大鼠分为正常对照组、模型组、Glu组和Glu＋锌原卟啉（ZnPP）组,每组8只.腹腔注射内毒素脂多糖（LPS）10 mg/kg制备内毒素血症动物模型.Glu组注射LPS前12 h灌胃Glu 1 g/kg;Glu＋ZnPP组注射LPS前12 h灌胃Glu 1 g/kg,注射LPS前1 h静脉注射ZnPP 10 mmol/kg.术后12 h取回肠组织,测定髓过氧化物酶（MPO）活性及肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）含量,并进行肠组织学评分;用免疫组化法检测回肠组织HO-1表达.结果 与正常对照组比较,模型组回肠组织学评分、MPO活性及TNF-α、IL-10含量显著升高[组织学评分（分）：3.3±0.4比1.1±0.6,MPO活性（U/g）：0.40±0.08比0.26±0.07,TNF-α含量（ng/g）：25.2±6.9比6.5±2.8,IL-10含量（ng/g）：27.6±10.2比5.7±2.9,均P〈0.01];HO-1表达较低.与模型组比较,Glu组组织学评分、MPO活性和TNF-α含量明显减低[组织学评分（分）：1.6±0.5比3.3±0.4,MPO活性（U/g）：0.25±0.05比0.40±0.08,TNF-α含量（ng/g）：13.4±3.2比25.2±6.9,均P〈0.01],IL-10含量（ng/g）显著升高（47.3±5.5比27.6±10.2,P〈0.01）,HO-1表达明显增加.Glu＋ZnPP组与模型组各指标比较差异无统计学意义.结论 Glu能明显增强内毒素血症大鼠肠组织HO-1表达,明显减轻肠道炎症反应,从而保护肠黏膜.
Objective To investigate the protective effect of glutamine （Glu） pretreatment on intestinal injury induced by endotoxin and expression of heme oxygenase-1 （HO-1） in rats.Methods Thirty-two male Sprague-Dawley （SD） rats were randomly divided into four groups （n=8 in each group）： normal control group, model group, Glu group and Glu＋zinc protoporphyrin （ZnPP） group. In model group, endotoxemia was produced by intraperitoneal injection of lipopolysaccharide （LPS, 10 mg/kg）. In Glu group, the rats received intragastraiclly 1 g/kg of Glu 12 hours before LPS intraperitoneal injection. In Glu＋ZnPP group, the rats received 1 g/kg of Glu by gavage 12 hours before LPS intraperitoneal injection and ZnPP 10 mmol/kg intravenously via tail vein 1 hour before LPS injection. The distal ileum was harvested in full thickness 12 hours after LPS injection. The myeloperoxidase （MPO） activity, tumor necrosis factor-α （TNF-α） and interleukin-10 （IL-10） in the intestine were determined, the pathologic changes were observed and expressed in Chiu grade. The expression of HO-1 was evaluated by immunohistochemistry method. Results Compared with normal control group, the Chiu grade, MPO activity, the content of TNF-α and IL-10 were significantly increased in model group [Chiu grade： 3.3±0.4 vs. 1.1±0.6, MPO activity （U/g）： 0.40±0.08 vs. 0.26±0.07, TNF-α （ng/g）： 25.2±6.9 vs. 6.5±2.8, IL-10 （ng/g）： 27.6±10.2 vs. 5.7±2.9, all P〈0.01], and the expression of HO-1 was decreased. Compared with model group, the Chiu grade, MPO activity, the content of TNF-α in Glu group were significantly decreased [Chiu grade： 1.6±0.5 vs. 3.3±0.4, MPO activity （U/g）： 0.25±0.05 vs. 0.40±0.08, the content of TNF-α （ng/g）： 13.4±3.2 vs. 25.2±6.9, all P〈0.01], while the level of IL-10 （ng/g） elevated （47.3±5.5 vs. 27.6±10.2, P〈0.01）, and the expression of HO-1 was increased. There was no difference in above mentioned indexes between model group and Glu＋ZnPP group. Conclusion Glu pretreatment significantly ameliorates the expression of HO-1 of intestinal tissue induced by LPS in rats, and intestinal mucosa is protected with alleviation of inflammatory reaction in intestinal tract.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2011年第2期95-98,共4页
Chinese Critical Care Medicine
基金
基金项目：江苏省研究生培养创新工程项目（CX09S-035Z）
江苏省徐州医学院院长人才专项基金（09KJZ07）
