摘要
目的:观察LED活化竹红菌素介导的光动力作用对人卵巢癌细胞生长的抑制作用。方法:采用倒置显微镜观察和MT法检测LED活化HB光动力作用后人卵巢癌细胞株HO8910细胞的生长状况。结果:LED活化HB介导的光动力作用显著抑制人卵巢癌细胞HO8910细胞的生长,在一定浓度的HB条件下,细胞生长抑制作用和LED能量密度间呈剂量依赖关系。倒置显微镜下观察到LED活化HB介导的光动力作用组癌细胞数量显著减少,细胞固缩,而单纯LED照射组、单纯HB光敏剂处理组和空白对照组组间无明显差异;MTT法示LED活化HB介导的光动力作用组细胞抑制率显著高于单纯LED照射组、单纯HB组和空白对照组(P<0.01),而单纯HB光敏剂处理组和空白对照组组间无明显差异(P>0.05).结论:LED可以作为一种新型的光源活化HB等光敏剂介导的光动力作用从而有效抑制人卵巢癌细胞株HO8910细胞生长。
Objective： To explore photedynamic inhibition of Lighting Emitting Diode （LED） - activated Hypocerllin B （HB） in human ovarian carcinoma cells. Methods： A special lighting emitting diode equipment was employed to investigate the pbotocytotoxie potency and efficacy of FIB 24h after PDT in a human ovarian carcinoma cell line HO8910 by inverted microscope and MTT reduction assay. Results： Inverted microscope and MTT reduction assay showed that the photodynamic inhibition of LED - activated HB in H08910 was extraordinarily significant. The photocytotoxieity of lIB indicated light - dose dependent characteristics in H08910 cells. Conclusion： Lighting Emitting Diode （LED） - activated HB could effectively kill human ovarian carcinoma cells and LED will beconre an ahemative light source for photodynamic therapy.
出处
《激光杂志》
CAS
CSCD
北大核心
2011年第1期64-65,共2页
Laser Journal
