摘要
目的：研究卡介苗细胞壁骨架（ＢＣＧＣＷＳ）加白细胞介素２（ＩＬ２）膀胱灌注对肿瘤组织端粒酶活性的影响，从而探讨该治疗的作用机理。方法：采用端粒重复序列扩增法（ＴＲＡＰ）分别检测２０例膀胱肿瘤用药前后端粒酶活性的表达。结果：经分别测定端粒酶提取原液以及１０倍、１００倍稀释液中端粒酶活性的表达，表明用药后肿瘤组织中端粒酶的活性明显降低（Ｐ＜００１）。结论：ＢＣＧＣＷＳ联合ＩＬ２膀胱灌注能有效地抑制肿瘤细胞端粒酶的活性，提示该治疗的作用机理之一可能是肿瘤端粒酶活性的改变。
Objective: To explore the influence on telomerase activity of bladder cancers after the treatment
of intravesical instillation with cell wall skeleton of bacillus CalmettGuerin(BCGCWS) and
interleukin2(IL2). Methods: the telomerase activity of tissues of 20 cases of bladder cancers
before and after the treatments with BCGCWS and IL2 was detected by telomeric repeat
amplification protocol(TRAP) respectively. Results: The telomerase activity in bladder cancer
was obviously reduced after the treatment of intravesical instillatios with BCGCWS and IL2
Conclusions:the telomerase activity of bladder cancers can be inhibited effectively by the use
of intravesical instillatios with BCGCWS and IL2, and the telomerase maybe a very important
signal in the treatments of bladder cancers.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第3期279-280,313,共3页
Chinese Journal of Cancer
基金
吴阶平泌尿外科基金
