摘要
目的：利用微生物酶转化由ＤＬ－肉碱消旋体制备Ｌ－肉碱。方法：ＤＬ－肉碱脱水制得巴豆甜菜碱，经大肠杆菌转化形成Ｌ－肉碱。薄层定性、半定量分析肉碱及其它季铵盐，酶旋光定量测定Ｌ－肉碱。结果：改进对甲苯磺酸法制备巴豆甜菜碱，用时短，产率由４８％提高到８０％以上；分析展开剂，用双向薄层层析分析发酵样品，得到良好效果；酶旋光测定法快速、精确、操作简便，减少了分析系统误差；厌氧、ｐＨ７．６，４０℃左右为游离酶和微生物生长细胞转化巴豆甜菜碱的有利条件。结论：建立了肉碱分析及微生物酶转化由ＤＬ－肉碱消旋体制备Ｌ－肉碱的方法。
OBJECTIVE:To produce L carnitine from DL carnitine.METHOD:To preprae crotonobetaine from DL carnitine by 3 dehydrated methods,then transform crotonobetaine to L carnitine by E.coli. The thin layer chromatography for carnitine and crotonobetaine and a simple enzymatic spectrophotometic assay for free carnitine were used.RESULTS:By modified ptoluene sulfonic acid method,the productivity increased from 48% to over 80%,The reaction time was shorten to 2.5h.Two directional thin layer chromatography was suitable for the assay of fermented sample.Carnitine enzymatically measurement method was rapid,accurate and convenient.Transcrotonobetaine produced L carnitine in both resting cells and growing cells.The optimum conditions were about 40℃,pH 7.6 and anaerobic.CONCLUSION:The methods to detect carnitine and the E.coli enzymatic transformational method to produce L carnitine from DL carnitine were developed.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1999年第5期335-337,共3页
Chinese Pharmaceutical Journal
