摘要
目的:建立RP-HPLC-UV法测定5种发酵虫草制剂百令胶囊、宁心宝胶囊、心肝宝胶囊、至灵胶囊及金水宝胶囊中麦角甾醇的含量。方法:色谱柱为Agilent SB TC C18(250 mm×4.6 mm,5μm),检测波长283 nm,流动相为甲醇-水(98:2),流速1.0 mL.min-1。结果:麦角甾醇进样量在0.42～6.29μg范围内呈良好的线性关系(r=0.9999)。平均加样回收率(n=3)分别为98.1%,100.7%,99.6%;RSD分别为2.3%,1.6%,1.6%。18批发酵虫草制剂中麦角甾醇含量,百令胶囊为6.06～6.10 mg.g-1,宁心宝胶囊为2.51～3.93 mg.g-1,心肝宝胶囊为3.47～4.42 mg.g-1,至灵胶囊为2.48～4.61 mg.g-1,金水宝胶囊为3.45～3.87 mg.g-1。结论:本文方法操作简便、灵敏,具有良好的重复性和稳定性,可用于发酵虫草制剂中麦角甾醇的检测。
Objective：To develop an RP-HPLC-UV method for the determination of contents of ergosterol in the preparations of fermental Cordyceps,such as Bailing capsule,Ningxinbao capsule,Xinganbao capsule,Zhiling capsule and Jinshuibao capsule.Method：The analysis was carried out on an Agilent SB TC C18（250 mm×4.6 mm,5 μm） column eluted with the mobile phase consisting of methanol-water（98∶2）.The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 283 nm.Results：The linear range of ergosterol was 0.42-6.29 μg（r=0.9999）.The average recoveries（n=3） were 98.1%,100.7%,99.6% and RSDs were 2.3%,1.6%,1.6% respectively.The contents of ergosterol in preparations of fermental Cordyceps were 6.06-6.10 mg·g-1（Bailing capsule）,2.51-3.93 mg·g-1（Ningxinbao capsules）,3.47-4.42 mg·g-1（Xinganbao capsules）,2.48-4.61 mg·g-1（Zhiling capsules） and 3.45-3.87 mg·g-1（Jinshuibao capsules）.Conclusion：The method is effective,sensitive and accurate with high repeatability and stability,which is helpful for the determination of ergosterol in the preparations of fermental Cordyceps.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第2期258-260,共3页
Chinese Journal of Pharmaceutical Analysis
基金
国家科技重大专项课题"中药质量标准研究和信息化体系建设平台"(2009ZX09308-004)专题内容
