摘要
目的:建立用超高效液相色谱(UPLC)法快速测定苏南山肚痛丸中芍药苷含量。方法:采用Acquity BEH C18(2.1 mm×50 mm,1.7μm)色谱柱,以乙腈-0.1%磷酸(15:85)为流动相,流速0.21 mL.min-1,检测波长230 nm,柱温25℃,进样体积2μL,采集频率20 Hz。利用Acquity Waters system进行含量测定。结果:样品中芍药苷在4.5 min内完全分离并对其进行了含量测定,芍药苷进样量在0.026～0.264μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率(n=6)为102.2%(RSD=0.4%)。结论:UPLC法在不影响分离效果的情况下大大提高了芍药苷分析速度,同时减少了溶剂消耗。该研究表明UPLC法可作为传统HPLC法的一种更为方便、可行的替代方法。
Objective：To establish a ultra-performance liquid chromatography（UPLC） method for determination of paeoniflorin in Sunanshandutong pills.Methods：The separation was performed on Acquity BEH C18（2.1 mm×50 mm,1.7 μm） column;The mobile phase was acetonitrile-0.1% Phosphoric acid（15∶85） at a flow rate of 0.21 mL·min-1;The detection wavelength was 230 nm,and the column temperature was 25 ℃;Injection volume was 2 μL;Collection frequency was 20 Hz.Acquity Waters system was used for the assay.Results：Paeoniflorin was completely separated and detected in 4.5 minutes;Linear range was 0.026-0.264 μg（r=0.9999）;Recovery（n=6） of method was 102.2%,RSD was 0.4%.Conclusion：The method is sensitive,accurate,precise,repeatable and suitable for determination of paeoniflorin in Sunanshandutong pills.Comparison to HPLC,UPLC obtain a faster analysis and less solvent consumption without reducing the efficiency.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第2期251-253,共3页
Chinese Journal of Pharmaceutical Analysis
