摘要
目的:利用多波长高效液相色谱法,建立消肿止痛酊中3种有效成分(栀子苷、丹皮酚、樟脑)的含量测定方法,考察该产品在有效期内不同生产日期的内在质量。方法:采用Waters SunFire C18(4.6 mm×250 mm,5μm)色谱柱,柱温30℃;甲醇(A)-水(B)为流动相,以1 mL.min-1流速梯度洗脱;检测波长:238 nm(0～14 min,检测栀子苷)、274 nm(15～32 min,检测丹皮酚)、288 nm(33～60 min,检测樟脑)。结果:在60 min内消肿止痛酊中栀子苷、丹皮酚、樟脑3种有效成分被完全分离,峰面积与其浓度呈良好的线性关系(r=0.9999);加样回收率(n=6):栀子苷为100.9%(RSD=0.6%),丹皮酚为100.2%(RSD=1.4%),樟脑为100.6%(RSD=1.0%)。结论:本方法操作简单,结果准确,为有效控制消肿止痛酊质量,提升质量标准,提供了一种可靠的检测方法。
Objective：To establish an HPLC with multi-wavelength detection method for simultaneous determination of geniposide,paeonol and camphor in Xiaozhong Zhitong tincture,and the quality of the tincture produced in different date was investigated using this developed method.Methods：The samples were separated on a Waters SunFire C18（4.6 mm×250 mm,5 μm） column,by a gradient elution using methanol（A） and water（B） as mobile phase at a flow rate of 1.0 mL·min-1 at 30 ℃;The eluate was detected by multiple wavelength：238 nm for geniposide（0-14 min）,274 nm for paeonol（15-32 min）,288 nm for camphor（33-60 min）.Results：The complete separation was obtained within 60 min for the three compounds（geniposide,paeonol and camphor）;Three regression equations showing linear relationships between peak-area and content of each compound were obtained.The average recoveries（n=6） of the three compounds were 100.9%（RSD=0.6%）,100.2%（RSD=1.4%）,100.6%（RSD=1.0%）,respectively.Conclusion：This method is simple,accurate,and provides a reliable detection methods for valid control of the product quality and improvement of quality standard.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第2期247-250,共4页
Chinese Journal of Pharmaceutical Analysis
