摘要
目的:建立清火栀麦片中栀子苷和穿心莲内酯、脱水穿心莲内酯的HPLC测定方法。方法:采用CLC-ODS C18(4.6 mm×150 mm,5μm)色谱柱;流动相为甲醇-乙腈-水,梯度洗脱,流速1.0 mL.min-1;检测波长:238 nm(0～10 min,栀子苷),224 nm(10.1～25 min,穿心莲内酯),250 nm(25.1～30 min,脱水穿心莲内酯)。结果:栀子苷、穿心莲内酯、脱水穿心莲内酯进样量分别在0.20～1.22μg、0.06～0.35μg、0.17～1.03μg范围内有良好的线性关系(r=0.9999)。平均回收率(n=6)分别为98.2%,97.8%,97.9%;RSD分别为0.74%,1.1%,1.0%。结论:该方法简便、快捷、准确,可用于该制剂的质量控制。
Objective：To establish an HPLC method for the determination of geniposide,andrographolide and dehydroandrographolide in Qinghuo Zhimai tablets.Methods：CLC-ODS C18（4.6 mm×150 mm,5 μm） column was adopted;The mobile phase consisted of methanol-acetonitrile-water with gradient elution at the flow rate of 1.0 mL·min-1;The detection wavelength was 238 nm（0-10 min） for geniposide,224 nm（10.1-25 min） for andrographolide,and 250 nm（25.1-30 min） for dehydroandrographolide.Results：The linear ranges of geniposide,andrographolide and was dehydroandrographolide were 0.20-1.22 μg,0.06-0.35 μg,and 0.17-1.03 μg,respectively.The average recoveries（n=6） of geniposide,andrographolide and was dehydroandrographolide were 98.2%（RSD=0.74%）,97.8%（RSD=1.1%）,and 97.9%（RSD=1.0%）,respectively.Conclusion：The method is simple,rapid,and can be used for the determination of three effective components in Qinghuo Zhimai tablets.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第2期228-230,共3页
Chinese Journal of Pharmaceutical Analysis
