摘要
为探讨改进大鼠肝脏低温保存方法对鼠肝的影响，即将切取后的鼠肝灌入一定量的自制保存液（ＨＹＤ液）后结扎进出肝脏的各血管。应用大鼠离体肝灌注模型，对传统保存法（对照组）和改进保存法（２０％组、３０％组及４０％组）肝脏微循环指标（门静脉灌注压、流出液内皮素１、台盼蓝分布时间及组织学改变）、流出液有关酶指标水平及分泌胆汁量进行了观察。结果：２０％组、３０％组及４０％组肝脏微循环指标及分泌胆汁量明显优于对照组（Ｐ＜０．０５），３０％组肝脏有关酶指标水平明显低于对照组（Ｐ＜０．０５），３０％组的保存肝脏效果最佳。本实验结果提示：改进保存法保存鼠肝的各项指标明显优于传统保存法，故本法行之有效，值得临床工作借鉴。
To investigate
the effect of modified cold preservation on rat livers. The modified method was that the
vascular bed of rat liver was infused with an additional 20 ml, 30 ml and 40 ml selfmade HYD
solution/100g liver. Via portal vein and the vesselses of liver were ligated. Forty rats were
randomly divided into four groups including conventional storage group (control group) and
modified preservation groups (20%, 30% and 40% group). The indices of hepatic
microcirculation, enzymes level in the effluent and bile production were determined in the
groups. Results: Bile production and all the indices of hepatic microcirculation including portal
perfused pressure, endothelin1 in the effluent, Trypan Blue distrubution time and histology in
experimental groups were significantly superior to those in control group (P<0.05). The liver
enzymes in 30% group were markedly lower than those in control group (P<0.05). The
preservation effect of rat livers in 30% group was the best among the experimental groups.
Conclusions: The modified cold storage method is effective and may have potential for clinical
application for liver preservation.
出处
《中国普外基础与临床杂志》
CAS
1999年第4期199-200,229,共3页
Chinese Journal of Bases and Clinics In General Surgery
基金
国家自然科学基金
黑龙江省青年基金
