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SDF-1/CXCR4生物学轴在乳腺癌中的表达及其临床意义 被引量：4

The Expression and Clinical Significance of the SDF-1/CXCR4 Biological Axis in Breast Cancer
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摘要 目的检测趋化因子基质细胞衍生因子1(SDF-1)及其受体CXCR4在乳腺浸润癌、导管原位癌及癌旁组织中的表达,分析两者与乳腺浸润癌临床病理学特征的关系,探讨SDF-1/CXCR4生物学轴在乳腺癌发生发展过程中的的作用。方法采用免疫组化方法检测80例乳腺浸润癌、15例导管原位癌和15例癌旁组织中SDF-1及CXCR4蛋白的表达。应用RT-PCR检测40例乳腺浸润癌、5例导管原位癌和5例癌旁组织中SDF-1及CXCR4 mRNA的表达。结果免疫组化检测结果显示,SDF-1、CXCR4在乳腺浸润癌与导管原位癌,导管原位癌与癌旁组织中的表达差异均有统计学意义(P<0.05),其中CXCR4表达与乳腺癌的淋巴结转移和分期有关(P<0.05)。RT-PCR检测结果显示,SDF-1与CXCR4 mRNA在乳腺浸润癌与导管原位癌,导管原位癌与癌旁组织中的表达差异均有统计学意义(P<0.05)。SDF-1、CXCR4蛋白及mRNA在乳腺浸润癌中的表达呈正相关(P<0.05)。结论 SDF-1/CXCR4生物学轴在促进乳腺癌的发生发展及侵袭转移过程中起促进作用。 Objective To analyze the clinicopathological significance of ligand SDF-1 and its receptor CXCR4 expression in breast carcinoma,ductal carcinoma in situ and adjacent tissues and clarify the roles of SDF-1/CXCR4 biological axis in the pathogenesis of breast cancer.Methods SDF-1 and CXCR4 protein expression were examined in 80 cases of breast carcinoma,15 cases of ductal carcinoma in situ and 15 cases of adjacent tissue by immunohistochemistry.SDF-1 and CXCR4 mRNA expression were determined in 40 cases of breast invasive carcinoma,5 cases of ductal carcinoma in situ and 5 cases of normal tissue adjacent to cancer tissues by RT-PCR.Results The protein and mRNA expressions of SDF-1 and CXCR4 were different in breast carcinoma and ductal carcinoma in situ,ductal carcinoma in situ and adjacent tissues（P 〈 0.05）.The expression of CXCR4 protein was related to lymph node metastasis and breast cancer stage（P 〈 0.05）.Both protein and mRNA expression of SDF-1 and CXCR4 were positively correlated in breast carcinoma（P 〈 0.05）.Conclusion SDF-1/CXCR4 biological axis might play an important role in promoting breast cancer growth and metastasis.
作者 宦大为 惠林萍 滕猛 王巧欢 张文竹 张弘
机构地区 沈阳医学院奉天医院病理科 中国医科大学基础医学院病理教研室 中国医科大学附属第四医院中心实验室
出处 《中国医科大学学报》 CAS CSCD 北大核心 2011年第2期166-171,共6页 Journal of China Medical University
关键词 SDF-1/CXCR4生物学轴 乳腺癌 免疫组化 RT—PCR SDF-1/CXCR4 biological axis breast carcinoma immunohistochemistry RT-PCR
分类号 R737.9 [医药卫生—肿瘤]
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参考文献9

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同被引文献40

  • 1王德辉.鼻内翻性乳头状瘤的鼻内镜手术[J].中国医学文摘（耳鼻咽喉科学）,2006,21(3):148-150. 被引量：25
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  • 7CHANG CC,CHEN SC,HSIEH YH,et al.Stromal cell-de-rived factor-1 but not its receptor,CXCR4,gene variants in-crease susceptibility and pathological development of hepatocel-lular carcinoma[J].Clin Chem Lab Med,2009,47(4):412-418.
  • 8吕海涛,王文斌,刘润田,等.CXCLl2与CXCR4在胆囊癌中的表达及其意义[J].中国老年医学杂志,2011,31(6):2113-2115.
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中国医科大学学报
2011年 第2期

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