摘要
针对甘薯叶片富含多糖、多酚、色素等物质的特点,以新鲜幼嫩甘薯叶片为材料,采用改良CTAB法提取基因组DNA,并对DNA进行电泳检测和AFLP分析。结果表明:使用改良CTAB法从32份甘薯叶片中提取基因组DNA,其纯度高、质量好、相对分子量大,多糖和多酚类等杂质去除比较完全,且OD260/OD230值>2.0,OD260/OD280值在1.8～2.0之间,无降解现象,能被限制性内切酶完全消化;用E-ACA和M-CTG引物组合进行AFLP分析,条带清晰,多态性好,此方法提取的甘薯叶片基因组DNA完全适于AFLP分析。
The leaf of sweet-potato is rich in polysaccharides,polyphenols and pigments,etc..The genomic DNA samples of young fresh leaves of sweet-potato were isolated by improved CTAB method,and then the agarose gel electrophoresis and the AFLP analysis were conducted to it.The results showed that each sample of the genomic DNA extracted from 32 leaf samples of sweet-potato by improved CTAB method has some good characters as follows： high purity,good quality,relatively high molecular weight,comparatively clean in removing polysaccharides and polyphenols;the value of OD260/OD230 was higher than 2.0,the value of OD260/OD280 was between 1.8 to 2.0;non-degradation,and it suitable for digestion by restriction enzyme.Using primer combination of E-ACA and M-CTG to do AFLP analysis,and the results showed the genomic DNA has clear bands and good polymorphism.Therefore,the genomic DNA,which is extracted from sweet-potato leaf by this improved CTAB method,is entirely suitable for the AFLP analysis.
出处
《湖南农业科学》
2011年第1期4-6,共3页
Hunan Agricultural Sciences
基金
国家甘薯产业技术体系(nycytx-16-c-3)
湖南省科技计划重点项目(2009nk2013)
