摘要
为了更好地了解禽源沙门菌对四环素的耐药性及耐药基因分布,从不同来源的家禽样品中分离沙门菌,调查其对四环素的耐药性以及耐药菌株中8种四环素耐药基因[tet(A)、tet(B)、tet(C)、tet(W)、tet(M)、tet(O)、tet(K)和tet(L)]的携带情况。结果表明,18.8%的沙门菌分离株对四环素耐药,健康成鸡分离株对四环素的耐药性明显高于雏鸡、死胚或病禽分离株;四环素耐药株中tet(A)、tet(B)和tet(M)基因的携带比例分别为73.1%、11.5%和3.8%,说明沙门菌对四环素的耐药机制以tet(A)和tet(B)基因介导的主动外排为主。本研究首次在对四环素耐药的沙门菌中检测到tet(M)基因,说明tet(M)基因在沙门菌对四环素的耐药性方面也具有潜在的作用。
In order to better understand antimicrobial susceptibility to tetracycline and distribution of tetracycline resistant genes of Salmonella in poultry,resistance to tetracycline of the isolats which were from poultry samples of different origins and 8 tetracycline resistance genes [tet （A ） ,tet （B） ,tet （C） ,tet （W）, tet （M） ,tet （0） ,tet （K）and tet （L） ] among tetracycline-resistant Salmonella isolates were detected. The results showed that 18.8% of isolates were resistant to tetracycline. Higher resistance rate was observed in Salmonella isolated from healthy adult chicken than chieklings,dead embryos and sick poultry. 73.1%,11.5% and 3.8% of tetracycline-resistant Salmonella harbored tet（A ）,tet（B）and tet（M） gene,respeetively,indicating active efflux mediated by tet（A ）and tet（B） genes as the primary mechanism of resistance to tetracycline in Salmonella. In this study,tet （M） gene was found in tetracycine-resistant isolates for the first time,which provided evidence concerning potential modes of tet（M）-conferred tetracycline resistance in Salmonella.
出处
《中国家禽》
北大核心
2011年第3期18-21,共4页
China Poultry
基金
北京市科技新星计划(2009B52)
2009-2010年度农业部动物源细菌耐药性监测计划
关键词
沙门菌
四环素
耐药性
四环素耐药基因
Salmonella
tetracycline
drug resistance
tetracycline resistance genes
