摘要
目的:研究孵育时间、二相性、pH环境对白色假丝酵母菌药物敏感性的影响。方法:参照美国临床实验室标准化委员会(NCCLS)推荐的M27-A方案的微量液基稀释法,测量5种常用抗真菌药物抗102株白色假丝酵母菌24h、48h的最低抑菌浓度(MIC)及在pH7.0和pH4.0环境下特比奈芬抗60株白色假丝酵母菌孢子相和菌丝相的MIC值。结果:(1)pH7.0环境下的孢子相液基中,咪康唑、伊曲康唑、克霉唑、氟康唑和制霉菌素24h与48h的MIC值基本符合率为91.2%～100%;(2)5种抗真菌药的药敏符合率均达80%以上。伊曲康唑24h与48h的药敏符合率稍低,为81.4%,其他4种药物药敏符合率为96.1%～100%;(3)pH7.0环境下,特比奈芬抗白色假丝酵母菌孢子相和菌丝相的MIC值差异无统计学意义(P=0.792),pH4.0环境下,孢子相液基的MIC值比菌丝相高,差异有统计学意义(P<0.001);(4)无论孢子相还是菌丝相液基,特比奈芬在pH4.0环境下的MIC值高于pH7.0环境,差异有统计学意义(P<0.001)。结论:孵育时间、二相性、pH环境对白色假丝酵母菌药物敏感性有影响。
Objective：To investigate the impacts of time,dimorphism and pH on antifungal susceptibility of candida albicans.Method：The 24h MICs and 48h MICs of miconazole,itraconazole,clotrimazole,fluconazole and nystatin for 102 isolates of candida albicans and the MICs of terbinafine for 60 candida albicans in two-form under pH7.0 and 4.0 were detected according to the broth microdilution methods proposed by the National Committee for Clinical Laboratory Standards（NCCLS）.Results：（1）In the base solution of yeast form at pH7.0,the essential agreement（EA） of MIC of miconazole,itraconazole,clotrimazole,fluconazole and nystatin were from 91.2% to 100% between 24h and 48h;（2）The broth microdilution results of categorical agreement（CA）of all the atifungal agents were above 80%.The CA of itraconazole was 81.4%,which is lower than 96.1%～100% of miconazole,clotrimazole,fluconazole and nystatin between the 24h and 48h（P0.001）;（3）When the pH was 7.0,the MICs of terbinafine against candida albicans showed no difference under the yeast and mycelial form（P=0.792）.When the pH was 4.0,the MICs of terbinafine against candida albicans in yeast form was significantly higher than that in mycelial form（P0.001）.（4）In the both yeast and mycelial form,the MICs of terbinafine against candida albicans under pH4.0 are significantly higher than that under pH7.0（P0.001）.Conclusion：The incubation time,dimorphism and pH affect the antifungal susceptibility of candida albicans.
出处
《现代妇产科进展》
CSCD
北大核心
2011年第1期30-34,共5页
Progress in Obstetrics and Gynecology
关键词
白色假丝酵母菌
孵育时间
二相性
PH
最低抑菌浓度
Candida albicans
Incubation time
Dimorphism
pH
Minimal inhibitory concentration
