摘要
目的:研究成人外周血间充质干细胞分化为神经元样细胞的潜能。方法:体外培养成人外周血中分离出的间充质干细胞,传代纯化后收集第2代细胞。细胞经预诱导及全反式维甲酸再诱导后,通过免疫细胞化学法鉴定诱导后的神经元样细胞。结果:诱导后外周血间充质干细胞分化为具有典型神经元形态的细胞,免疫细胞化学显示细胞神经元烯醇化酶阳性率68%;细胞神经巢蛋白阳性率55%;细胞胶质纤维酸性蛋白表达均阴性。结论:成人外周血间充质干细胞在体外可诱导分化为神经元样细胞。
Objective： To explore differentiation potentiality of human peripheral blood-derived mesenchymal stem cells（PBMSCs）into neuron like cells in vitro.Methods： PBMSCs were isolated from human peripheral blood through density gradient and then amplified.The second passage cells were collected and preinduced,and then induced with ATRA.Neuron like cells were detected by immunocytochemistry.Results： PBMSCs could be differentiated into cells with typical neuronal morphology,68% of the cells expressed NSE,55% of the cells expressed Nsetin,and none expressed GFAP.Conclusion： PBMSCs can be induced to become neuron like cells.
出处
《交通医学》
2010年第6期602-604,共3页
Medical Journal of Communications
基金
深圳市科技计划项目(200602040)
