摘要
【目的】探讨血管紧张素Ⅱ（AngⅡ）受体拮抗剂对博莱霉素A5（BLMA5）所致肺损伤的保护作用。【方法]24只SD雄性大鼠随机分为对照组（C组）、模型组（M组）和缬沙坦组（T组）三组，每组8只。T组气管内滴注BLMA5生理盐水溶液（5mg／kg）以复制急性肺损伤动物模型，并于造模当天每日给予缬沙坦（20mg／kg）灌胃；M组以生理盐水代替缬沙坦灌胃；C组则均用生理盐水代替BLMA5和缬沙坦。各组动物均于制模开始后d，处死，分取肺纽织行病理切片HE染色观察肺损伤程度、免疫组化技术检测肺组织转化生长因子β（TGF—β）的表达水平。【结果】T组的肺系数、急性肺损伤程度及TGF—β1在肺组织中的表达水平均显著低于M组。【结论】缬沙坦可减轻BI，MA5诱导的大鼠肺损伤程度。
[Objective] To explore the protective effect of angiotensin Ⅱ（Ang Ⅱ） receptor antagonist, val sartan on bleomycetin A5（BLM AS） induced lung injury in rats. [Methods]Twenty four SD male rats were randomly divided into control group（group C）, model group（group M） and valsartan group（group T） with 8 in each. Group T received intratracheal instillation of 5mg/kg BLM A5 normal saline solution to copy acute lung injury rat model, and then was given 20mg/kg valsartan by lavage everyday. Group M was given normal saline solution by lavage instead of valsartan. Group C was given normal saline solution instead of BLM A5 and valsartan. All animals in each group were killed at 7th day after establishing the model. The lung tissues were harvested for HE staining of pathological section in order to observe lung injury. Immunohistoehemistry method was used to detect the expression of transforming growth facto β （TGF-β1） in lung tissue. [Results] The lung coefficient, the degree of acute lung injury and the expression of TGF-β1 in lung tissue in group T were obviously lower than those in group M. [Conclusion] Valsartan can reduce the degree of BLM A5-induced lung injury in rats.
出处
《医学临床研究》
CAS
2010年第12期2237-2240,共4页
Journal of Clinical Research
