摘要
目的 研究酵母RAD2 4基因区域温度敏感突变株校正表型 ,克隆野生型人类同源基因。方法 用互补试验观察突变株rad2 4LR表型 ,以人的cDNA表达文库转化突变株 ,筛选回复表型。结果 RAD2 4L能校正突变株辐射敏感表型。以人cDNA表达文库转化rad2 4LR ,从转化文库后的回复表型中克隆出人类同源基因cDNA ,克隆的人类RAD2 4L同源基因cDNA蛋白质为 670个氨基酸 ,含有一般认定的解螺旋结构域GKT。结论 克隆了人类DNA修复基因hR2 4L,它能校正突变株的紫外敏感和X线敏感表型 ,其产物参与DNA的切除修复和重组修复。
Objective To study the correction phenotype of temperature sensitive mutant rad24LR of S cerevisiae and to clone wild human functional homologue. Methods The temperature sensitive mutant rad24LR was studied by complementation test Transformation of rad24LR was performed with human cDNA expression library to screen for reversion phenotype Results RAD24L can complement the radiation sensitive phenotype of the mutation. Its homologue of human cDNA was cloned by screening transformed rad24LR with human expression library for reversion phenotype Conclusion Human DNA repair gene hR24L was successfully cloned in this study. The product hR24L is involved in both DNA excision repair and recombination repair
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第12期941-943,共3页
National Medical Journal of China
基金
国家自然科学基金! 3 9770 40 9
国家 86 3计划生物技术领域青年基金
