摘要
目的 探讨黄芪当归合剂降低肾病综合征（ＮＳ）大鼠血脂的机制。方法 采用加速型肾毒血清肾炎致ＮＳ模型。治疗组灌服黄芪当归合剂，正常组和肾病对照组灌水。检测各组血清脂谱，以Ｎｏｒｔｈｅｒｎ 杂交检测肝脏羧甲基戊二酰辅酶Ａ（ＨＭＧＣｏＡ） 还原酶和低密度脂蛋白受体（ＬＤＬＲ）ｍＲＮＡ表达。结果 肾病大鼠呈高脂蛋白血症，该组肝脏ＨＭＧＣｏＡ还原酶的ｍＲＮＡ在早期呈短暂上调（ Ｐ ＜ ０－００１） ，ＬＤＬ受体ｍＲＮＡ 表达随病程逐渐下调（ Ｐ ＜ ０－００１）；黄芪当归治疗组，血清胆固醇、甘油三酯、低密度脂蛋白和极低密度脂蛋白低于肾病对照组，虽肝脏ＨＭＧＣｏＡ 还原酶ｍＲＮＡ 无明显变化，但ＬＤＬ受体ｍＲＮＡ表达较肾病对照组显著上调（ Ｐ＜ ０－０１） 。结论 黄芪当归组显著降低ＮＳ大鼠血脂水平，这一改善脂代谢紊乱的作用可能部份是通过肝脏ＬＤＬ受体ｍＲＮＡ 表达上调而实现。
Objective To find out the mechanism of Astragalus and Angelica (A & A) decreasing serum lipid in rats with nephrotic syndrome (NS). Methods A rat model of accelerated nephrotoxic serum nephritis with NS was used in this study. NS rats were divided into two groups: one group was treated with A & A, another group with water. In addition, normal rats were used as control. Serum lipid profile was measured by enzymological techneque in all three groups. Liver tissue HMG CoA reductase mRNA and LDL R mRNA expression were analysed by Northern bolt.Results Hyperlipidemia was observed in nephrotic rats. In this group, hepatic HMG CoA reductase mRNA expression was temporarily higher in 3rd day, LDL R mRNA expression was gradually down regulated (P<0 001,compared with normal control). Though liver tissue HMG CoA reductase mRNA had no obvious change in A & A group, the LDL R mRNA expression was significantly up regulated compared with NS group (P<0 01). Meanwhile, Ch、TG、LDL and VLDL were obviously lower in A & A group than that in NS group. Conclusion A & A can significantly decrease the serum lipid level in NS rats.This improvement of lipid metabolism disorder in NS rats may be made partly via up regulating LDL R mRNA expression in liver.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
1999年第6期337-339,共3页
Chinese Journal of Nephrology
基金
国家自然科学基金!(No .39670909)
