摘要
目的 了解上海地区流感嗜血杆菌（Ｈｉ）感染株、携带株的血清型及主要血清型的β内酰胺酶产生率。方法 收集急性上、下呼吸道感染患者的痰及咽拭子等标本５３４ 份及无呼吸道感染对照组咽拭子标本２５５ 份，应用改良哥伦比亚巧克力培养基进行分离培养；应用玻片凝集法及反向间接血凝法对分离出的Ｈｉ 进行血清分型，并检测Ｈｉｂ 和不可分型株（ＮＴＨｉ） 产β内酰胺酶情况。结果患者组标本中共分离出Ｈｉ１６４ 株，分离率为３１％ （１６４／５３４） 。其中Ｈｉｂ ６９ 株，占４２％ （６９／１６４） ，ＮＴＨｉ９１株，占５６％ （９１／１６４）。对照组共检出Ｈｉ ６６ 株，检出率为２６％ （６６／２５５），其中Ｈｉｂ 为１４ 株，占２１％（１４／６６） ，ＮＴＨｉ４９ 株，占７４ ％（４９／６６）。患者组１６０ 株Ｈｉｂ 和ＮＴＨｉ 共产β内酰胺酶３８ 株，二者共同阳性３６ 株，Ｈｉ 产酶率２３ ％（３６／１６０）；Ｈｉｂ 产酶阳性率为２９％ （２０／６９） ，ＮＴＨｉ 产酶阳性率为２０ ％ （１８／９１）。有１６ 株对氨苄西林耐药的菌株不产β内酰胺酶。结论 Ｈｉ 的血清型在患者组和对照组均以Ｈｉｂ 和ＮＴＨｉ 为主，Ｈｉｂ 分离率患者组高于对照组。Ｈｉｂ 产酶率高于?
Objective To investigate the serotypes of both infectious and carriage strains of Haemophilus influenzae (Hi)and to evaluate the prevalence of β lactamase of major serotypes in Shanghai Methods The 534 specimens of sputum and swabs from patients with acute upper or lower respiratory tract infection and 255 cases of control group were collected and incubated on improved Collumbia chocolate medium All isolated Haemophilus influenzae strains were serotyped by slide agglutinating assay or reverse indirect blood agglutinating method β lactamase were measured by iodine test and paper disc method Results In the patient group, of the 164 Hi strains there were 69 Hib strains (42%,69/164) and 91 NTHi(56%,91/164) In the control group,there were 14 strains of Hib and 49 strains of NTHi among 66 strains of Hi,which were isolated from 255 specimens(26%,66/255). 24%(38/160) of Hi in the patient group produced β lactamase In this group,the positive rate of β lactamase was 29%(20/69) for Hib strains and 20%(18/91) for NTHi strains Sixteen strains resisting to ampicillin were β lactamase negative Conclusions These results indicate that Hib and NTHi are the major serotypes of Hi in both the patient and control group The prevalence of Hib is higher in patients than in the control Hib strains are more likely to produce β lactamase than NTHi
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1999年第12期713-716,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
流感嗜血杆菌
感染率
产酶率
分型
Haemophilus influenzae Infection rate Carriage rate Serotype β lactamase
