摘要
目的观察大鼠肠缺血-再灌流损伤时肠粘膜通透性的变化及对血浆脂多糖（LPS）和脏器功能的影响。方法雄性Wistar大鼠，根据夹闭肠系膜上动脉的时间及松夹后的时间随机分9组。于实验各时相点采集标本，血浆D-乳酸和LPS分别用酶偶联法及偶氛显色鲨试验法检测。结果（1）肠缺血1～1．5小时，血浆LPS和D-乳酸开始升高，再灌流后显著升高，达峰值再灌流后0．5～1小时，2～6小时恢复正常；（2）血浆LPS和D-乳酸变化趋势一致，二者呈正相关（r=0．7198，P＜0．05）；（3）再灌流后小肠绒毛脱落，固有层白细胞聚集；（4）肝、肾、心功能失常，与LPS的变化趋势一致。结论肠缺血再灌流早期肠粘膜已受到严重损伤，通透性增加，导致LPS大量人血，脏器功能损伤。
Ojective To invesigate the change in intestine mucosal permeability following intestine ischemia-reperfusion injury in rat. Methods Wistar rats were divided into nine groups: intestine ischemia for 0. 5, 1 and 1.5h, intestine ischemia for 1h and subsequent reperfusion for 0.5, 1, 2, 6 and 24h. Blood Samples were obtained at various intervals. Plasma D-lactate and LPS concentrations were determined. Results The plasma concentration of D-lactate and LPS increased gradually at 1 ~ 1.5h post ischemia and increased markedly after reperfusion. At 2~6h post-reperfusion, the plasma D-lactate and LPS concentrations recovered to normal repectively. In addition, it was noted that there was a signficant correlation between the plasma concentrations of LPS and D-lactate (r = 0.7198, P<0.05). The desquamation of intestine villi and infiltration of inflammatory cell in the lamina propria were observed after reperfusion. The changes of organ function including liver, heart and kindey were parallel to the peak level of plasma LPS. Conclusion The data indicated that the intestine mucosal permeability was impaired during the early stage of intestine ischemia-reperfusion,which resulted in intestine derived endotoxamia and organ disfunction.
出处
《创伤外科杂志》
1999年第4期208-210,共3页
Journal of Traumatic Surgery
关键词
肠缺血
再灌注损伤
肠粘膜通透性
Intestine ischemia-reperfusion D-lactate Lipopolysaccharide
