摘要
利用高效液相色谱(HPLC),对刺参Apostichopus japonicus体腔注射大黄酸(Rhein)后的药代动力学进行了研究。以5.33mg/kg的剂量给刺参体腔注射大黄酸,测定大黄酸在其体腔液、呼吸树、肌肉、体壁中的药物浓度-时间变化。结果表明,大黄酸在上述4种组织中的达峰时间(Tmax)分别为0.26、0.67、0.54、0.88h;消除半衰期(T1/2β)分别为6.24、26.1、71.48、8.93h;药时曲线下总面积(AUC)分别为69.29、105.6、132.38、20.99mg/L.h。除体壁中的大黄酸代谢规律符合一级吸收一室模型外,大黄酸在其他3种组织中均符合一级吸收二室模型。以上研究表明,体腔注射大黄酸后,药物在刺参体内吸收快,在各组织中能迅速达到峰值,消除半衰期长,代谢慢,主要经呼吸树排出体外。
Studies were conducted on the pharmacokinetics of rhein in Apostichopus japonicus.Coelomic fluid,respiratory trees,muscle,and body wall were sampled after coelomic injecting with a single dose of rhein（5.33 mg/kg）.The contents of rhein were determined by high performance liquid chromatography（HPLC）.The results showed that the peak time（Tmax）,the elimination half-time（T1/2β）,and the area under the concentration-time curve（AUC）in coelomic fluid,respiratory trees,muscle,and body wall were as follows：Tmax：0.26,0.67,0.54 and 0.88 h;T1/2β：6.24,26.1,71.48,and 8.93 h;AUC：69.29,105.6,132.38,and 20.99 mg/L·h.The concentration-time course of rhein in coelomic fluid,respiratory trees,and muscle could be described by a two-compartment model,except for the course in body wall being described by a one-compartment model.These findings indicated that rhein can reach the peak time with a high speed absorption in A.japonicus after injection,while it is slowly metabolized with a long elimination half-time.Rhein is mainly excreted from respiratory trees.
出处
《渔业科学进展》
CSCD
北大核心
2010年第6期76-81,共6页
Progress in Fishery Sciences
基金
"十一五"国家科技支撑计划项目(2006BAD09A01)
海洋公益性行业科研专项经费项目(2009418020)共同资助
