摘要
研究了浸浴给药方式下,亚硝酸盐在鲫鱼Carassius auratus体内的药物动力学和残留情况,同时研究了Cl-、Br-对鲫鱼吸收NO2-离子的影响。结果显示,在18±2℃水温条件下,以60mg/L亚硝酸盐水体浸浴10d后,其血药浓度-时间数据用一级吸收二室模型描述较为合适,血浆和肌肉的主要药动学参数:t1/2α、t1/2β、AUC及CL(s)分别为0.92d、1.50d;5.36d、7.98d;0.72d.mg/L(kg)、1.10d.mg/L(kg);83.1mg/L(kg).d、54.7mg/L(kg).d。线性回归系数r=0.9999,方法检出限为0.01mg/kg,回收率为78.0%~93.7%,相对标准偏差为4.88%~9.17%。肌肉作为可食性组织,且消除最慢,因此选取肌肉组织作为残留检测的靶组织,以0.02mg/kg为最高残留限量,在(18±2)℃水温条件下,10d后亚硝酸盐代谢完全。检测结果表明,加入氯化钠、溴化钠后各组织亚硝酸盐的含量前者最低,对照组最高。结果表明,Cl-对NO2-吸收影响程度比Br-高,氯、溴离子可以减轻亚硝酸盐的毒害性。
Pharmacokinetics and residues of nitrite in Carassius auratus were investigated in this study.Also,the impact of chlorine and bromine ion on nitrite adsorption by C.auratus was studied.C.auratus was exposed to nitrite at concentration of 60 mg/L in bath water for 10d at 18±2℃.The concentration of the drug in the serum and muscle was described as a two compartmental open model,and the main pharmacokinetics parameters t1/2α,t1/2β,AUC,and CL（s）were 0.92d,1.50d;5.36d,7.98d;0.72 d·mg/L（kg）,1.10 d·mg/L（kg）;83.1mg/L（kg）·d,54.7 mg/L（kg）·d,respectively.The limit of quantification（LQ）was 0.01mg/kg.The linear plots were obtained with linear regression coefficient（r）of 0.999 9.Average recoveries were between 78.0%～93.7% with RSD values between 4.88%～9.17%.According to the maximum residue limit（MRL）of 0.02 mg/kg in muscle,the nitrite would be metabolized completely after ten days in C.auratus at the water temperature of 18±2 ℃.The other results showed that the content of nitrite in the three tissues of treatments with both nitrite and NaCl or NaBr added were lower than the nitrite-only treatments.The lowest nitrite values were detected in treatments with NaCl.The results indicate that chlorine and bromine ion are capable of reducing nitrite toxicity.
出处
《渔业科学进展》
CSCD
北大核心
2010年第6期29-35,共7页
Progress in Fishery Sciences
基金
国家质量监督检验检疫总局科技计划项目(2006IK104)资助
