摘要
参照鸭GH基因序列设计引物扩增松辽白鹅GH基因外显子2和内含子2的部分序列,并运用PCR-SS-CP技术进行多态性分析。结果表明:此扩增片段存在多态性,得到AA、AB和BB等3种基因型。3个品种中等位基因A占绝对优势,且主要以AA型存在。在GH基因外显子2第2301处发生了单碱基的突变(C→T),内含子2第2479处发生了单碱基突变(T→C)。方差分析结果表明:不同基因型的宰前活重、屠体重、半净膛重、全净膛重、腹脂重、头重以及屠宰率性状间有显著差异,其它大部分生长和屠体性状不显著。
A part of GH gene exon 2 and intron 2 sequence of Songliao goose was designed according to GH gene sequence of duck. The polymorphism of those genes was analyzed by PCR-SSCP. The results showed as follows：first of all,the amplified fragment possessed polymorphism including AA,AB and BB,three genotypes. Allele A held absolutely dominance in three different species of goose. Secondly,the single base was changed on 2301st spot of exon 2 of GH gene（C→T）,and on 2479th spot of intron 2 of GH gene（T→C）.The results of significance test showed that living weight,carcass weight,half-eviscerated weight,whole-eviscerated weight,abdominal fat weight,head weight and dressing percentage were significantly different among different genotypes,and others showed no difference.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2010年第6期1240-1244,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
吉林省财政厅资助项目
