摘要
研究了中药茜草根中分离出具有较高抗氧化活性的组分.主要采用吸附柱层析法,经不同的洗脱体系,从茜草根乙酸乙酯萃取物(I)中分离得到较纯组分(VAGJT);运用硅胶薄层色谱(TLC)及高效液相色谱(HPLC)对该组分的化学成分进行分析;并采用铁离子还原(FRAP)法,硫代巴比妥酸(TBA)法和二苯代苦味酰基自由基(DPPH)法对VAGJT及茜草根乙酸乙酯萃取物(I)的抗氧化活性进行测定.结果表明:组分VAGJT的TLC结果显示只有一个斑点,HPLC结果有三个强峰且保留时间相差较小;VAGJT及茜草根乙酸乙酯萃取物(I)的抗脂质过氧化能力均高于抗坏血酸(Vc),且VAGJT清除自由基能力及铁还原能力均高于茜草根乙酸乙酯萃取物(I).
The purpose of this project is the separation of components with high antioxidant activity from Rubia cordifolia L.A component（VAGJT）was separated from the ethyl acetate extraction of Rubia cordifolia L.by absorption column chromatography with different wash-solvent systems.The chemical composition and contents of VAGJT were analyzed by thin-layer chromatography（TLC）and high-performance liquid chromatography（HPLC）.The antioxidant activity of VAGJT and the ethyl acetate extraction of Rubia cordifolia L.（I）were also evaluated by Ferric Reducing Antioxidant Power（FRAP）,2-thiobarbituric acid（TBA）and 1,1-pheny-2-picrylhydrazyl（DPPH）assays.Only one spot was presented in TLC analysis of VAGJT,and three strong peaks were displayed in HPLC.The results showed that the anti-lipid peroxidation activity of VAGJT and the ethyl acetate extraction of Rubia cordifolia L.（I）were higher than that of ascorbic acid（Vc）,and the anti-free radical scavenging ability and restoring the ability of ferrous ions of VAGJT were higher than that of the ethyl acetate extraction of Rubia cordifolia L.（I）.
出处
《武汉工程大学学报》
CAS
2010年第12期1-5,共5页
Journal of Wuhan Institute of Technology
基金
农业部热带农林有害生物入侵监测与控制重点开放实验室开放课题基金专项资助项目(MACKL1006)
武汉市科技局对外科技合作与交流计划(200970634272)
绿色化工过程省部共建教育部重点实验室开放研究基金(GCP200906)
武汉工程大学大学生校长基金
关键词
茜草根
乙酸乙酯萃取物
分离
洗脱体系
抗氧化活性
Rubia cordifolia L
ethyl acetate extraction
separation
wash-solvent system
antioxidant activity
