摘要
目的:探讨MS-275与化疗药物顺铂(cisplatin,DDP)联合应用对肝癌细胞系HLE增殖的抑制效果。方法:通过MS-275联合化疗药物顺铂处理培养的肝癌细胞系HLE,体外细胞毒性实验(MTT)法检测细胞增殖抑制率,流式细胞术(FCM)检测细胞周期和凋亡率。结果:MTT结果显示:0.5μmol/LMS-275与6.25μg/ml DDP联合应用后5d,HLE细胞增殖抑制率达(97.86士5.62)%,明显高于单用MS-275组的(51.75±2.31)%和DDP组的(43.48士2.39)%(P<0.05)。流式细胞术结果显示:MS-275与DDP联合应用明显导致细胞S期减少,G_2/M期阻滞;MS-275联合DDP组细胞凋亡率为(17.46士2.57)%,明显高于单用MS-275组的(7.62士0.59)%和DDP组的(7.36±0.47)%(P<0.05)。结论:MS-275与DDP联合应用能显著提高对肝癌细胞系HLE增殖的抑制作用。
Objective:To investigate the inhibitory effect of MS-275 combined with cisplatin(DDP) on growth of human hepatocellular carcinoma cell line HLE.Methods:Human hepatocellular carcinoma cell line HLE was treated with MS- 275 combined with DDP.The growth inhibition rate of cells was analyzed by MTT assay,the cell cycle and apoptosis were detected by flow cytometry.Results:Five days after combined treatment with MS-275(0.5μmol/L) and DDP (6.25μg/ml),the growth inhibitory rate was(97.86±5.62)%,which was significantly higher than those in MS-275 group(51.75±2.31)%(P<0.05) and DDP group(43.48±2.39)%(P<0.05).Flow cytometry displayed that combined administration of MS-275 and DDP remarkably reduced S phase and arrested HLE cells at G_2/M phase.The cell apoptotic rate was(17.46±2.57)%in MS-275 and DDP group,which was significantly higher than those in MS-275 group(7.62±0.59)%(P<0.05) and DDP group(7.36±0.47)%(P<0.05).Conclusion:MS-275 combined with DDP has enhanced inhibitory effect on growth of human hepatocellular carcinoma cell line HLE.
基金
大学生创新性实验项目(国家级)(091048930)
关键词
肝癌
细胞系
MS-275
顺铂
基因治疗
Hepatocellular carcinoma
Cell line
MS-275
Cisplatin
Gene therapy
