摘要
确定分光光度法测定酸奶中钙含量的最佳操作条件。在pH值为10.8的NH3-NH4Cl缓冲溶液介质中,在溴化十六烷基吡啶(CPB)的作用下,Ca2+与二甲酚橙(XO)生成蓝色配合物,最大吸收波长为620nm,表观摩尔吸光系数为6.7×104L.mol-1.cm-1,Ca2+含量0～15μg/25mL范围内符合比尔定律,相对标准偏差≤3.1%,加标回收率为96%～101.6%。方法具有灵敏度高,准确度好、体系稳定、试剂和仪器廉价、操作简便快速等优点。
The aim was to determine the best operating conditions in measuring the content of calcium in yoghurt by spectrophotometry.in the NH3-NH4Cl buffer medium at pH 10.8 and in the presence of cetylpyidiumbromid,orange reacts with calcium to form a blue complex with a maximum absorption peak at 620 nm.The apparent molar absorptivity of the complex is 6.7×104 L·mol-1·cm-1.Beer＇s law is obeyed for the concent ration oftitanium in the rang of 0~15μg/ 25 mL.Relative standard deviation forcalcium determination was less than 3.1%,The recovery was 96%~101.6%.Method had high sensitivity and had advantages of good stability,high accuracy,cheap reagent and instruments as well as simple and rapid operation.
出处
《巢湖学院学报》
2010年第6期77-79,共3页
Journal of Chaohu University
