摘要
目的探讨慢性乙型肝炎(CHB)患者外周血CD4+CD2+5调节性T细胞(tregulatory cell,Treg)、转化生长因子β1(transforminggrowth factorβ1,TGF-β1)水平变化及临床意义。方法选择105例CHB患者为病例组,其中依患者病情分为轻、中、重度组;选择26例健康体检正常者为对照组。用流式细胞仪检测外周血中Treg表达水平;用双抗夹心ELISA法检测血清TGF-β1;用PCR法检测患者HBV-DNA载量。结果 CHB患者组Treg、TGF-β1水平明显高于对照组,差异均有统计学意义。Treg水平与TGF-β1水平呈正相关。结论 CHB患者Treg、TGF-β1表达增加,提示Treg、TGF-β1在CHB中担负着重要的免疫调节作用,可能抑制CHB患者特异性细胞免疫反应,与HBV感染的慢性化及肝病的临床发生、发展有关。
Objective To explore the expression level of Treg and transforming growth factor β1（TGF-β1） in the peripheral blood of patients with chronic hepatitis B virus（CHB） infection and its clinical significance.Methods 105 patients with CHB were selected,and they were divided into three groups according to the state of illness.26 healthy people were selected as control group.Treg,TGF-β1 and the level of HBV-DNA in serum were detected by flow cytometry,ELISA and PCR respectively.Results The levels of Treg and TGF-β1 in CHB group were significantly higher than that of control group.The level of Treg was positively correlated with the level of TGF-β1.Conclusion The expression levels of Treg and TGF-β1 both increase in CHB patients,which indicates that Treg and TGF-β1 plays an important role in immunoregulation in CHB patients as well as in preventing specific cell immune response.It may be related to the chronic turn and the occurrence and development of hepatic disease.
出处
《中国全科医学》
CAS
CSCD
北大核心
2010年第36期4066-4068,共3页
Chinese General Practice
