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肽段连接的近红外荧光量子点对人颊鳞癌BcaCD885细胞侵袭和转移能力的影响 被引量：1

Study on effect of peptide-conjugated near-infrared fluorescent quantum dots on invasion and metastasis of human buccal squamous cell carcinoma cell line BcaCD885
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摘要 目的研究肽段连接的近红外荧光量子点(QDs)对人颊鳞癌BcaCD885细胞的生长、侵袭、黏附和趋化运动能力的影响。方法 1)用表面连接穿膜肽段最大发射波长为800 nm的近红外荧光量子点(QD800)标记BcaCD885细胞(BcaCD885/QD800),用流式细胞仪检测QD800对BcaCD885细胞的标记率,用激光扫描共聚焦显微镜观察QD800在BcaCD885细胞内的分布情况;2)用不同浓度的QD800与BcaCD885细胞共培养,观察QD800对BcaCD885细胞生长的影响;3)用Transwell侵袭小室法和冲刷实验,比较BcaCD885/QD800和BcaCD885细胞侵袭、黏附和趋化运动能力的变化。结果 1)QD800对BcaCD885细胞标记后6 h时的标记率为94.07%,QD800主要分布在细胞质内;2)不同浓度的QD800均未影响BcaCD885细胞的生长情况;3)BcaCD885/QD800和BcaCD885细胞的侵袭、黏附和趋化运动能力无显著性差异(P>0.05)。结论用肽段连接的近红外荧光量子点标记BcaCD885细胞后不影响其生长、浸润和转移能力,为利用量子点进一步在活体条件下非侵入的体内肿瘤细胞成像和示踪研究提供了科学依据。 Objective To study the effect of peptide-conjugated near-infrared quantum dots（QDs） on growth, invasion and metastasis of human buccal squamous cell carcinoma cell line（BcaCD885 cell）.Methods 1）BcaCD885 cells were labeled by cell-penetrating peptide-conjugated QDs with a maximum emission wavelength of 800 nm（QD800）,then labeling efficiency was detected by flow cytometry,and laser-scanning confocal microscope was used to observe the distribution of QD800 within the cells.2）Different concentrations of QD800 was applied to BcaCD885 cells,and the cell growth of control and three test groups were compared respectively.3）BcaCD885 cells were labeled by QD800（BcaCD885/QD800）,then transwell chambers and wash way were used to dectect the difference of invasion and metastasis ability between BcaCD885/QD800 and BcaCD885 cells.Results 1）The labeling rate of BcaCD885 cells after 6 h was 94.07%,and QD800 distributes in the BcaCD885 cytoplasm.2）Different concentrations of QD800 showed no negative effects on growth of BcaCD885 cells.3）The ability of invasion,attachment and motion of BcaCD885 cells were not significantly different between test and control group（P0.05）.Conclusion QDs showed no effects on growth,invasion and metastasis ability of BcaCD885 cells.Our results provide science foundation for QDs as a new fluorescence probes to real-time monitor cells and cells imaging in a living.
作者 梅杰 杨凯 李志刚 曹雨庵
机构地区 重庆医科大学附属第一医院口腔颌面外科
出处 《华西口腔医学杂志》 CAS CSCD 北大核心 2011年第1期92-95,共4页 West China Journal of Stomatology
基金 国家自然科学基金资助项目(30872925和30470893)
关键词 量子点 近红外荧光 黏附 侵袭 quantum dots near-infrared fluorescence attachment invasion
分类号 R739.8 [医药卫生—肿瘤]
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参考文献12

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同被引文献13

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华西口腔医学杂志
2011年 第1期

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