摘要
目的探讨不同类型一氧化氮合酶在慢传输型便秘(STC)大鼠结肠的表达。方法健康Wistar大鼠32只,随机分为STC组、对照组,每组16只。采用复方苯乙哌啶灌胃法制备STC大鼠模型,饲养100天后,采用活性炭灌胃法测定肠道传输速度确定模型建立。用免疫组织化学方法分别检测诱导型一氧化氮合酶(iNOS)、神经型一氧化氮合酶(nNOS)及内皮型一氧化氮合酶(eNOS)在大鼠结肠的表达情况。结果 STC组大鼠肠道传输速度与对照组相比明显减慢,首粒黑便排出时间为(686±57)min,较对照组大鼠(608±46)min显著延长(P<0.05)。免疫组化结果显示,iNOS在STC大鼠结肠的表达明显强于对照组(P<0.05);eNOS、nNOS的表达与对照组比较均无明显差异(P>0.05)。结论 iNOS在STC大鼠结肠的表达异常,表明iNOS在慢传输型便秘发病机制中可能起重要作用。
Objective To investigate the expression of different kinds of nitric oxide synthase（NOS）in the colon of slow transit constipation（STC） rats. Methods Thirty-two healthy Wistar rats were randomly divided into control group and model group of slow transit constipation.The rats in model group received daily diphenoxylate by intragastric administration to develop the slow transit constipated model.After 100 days,intestinal transit functions were examined by activated charcoal pushing test.Meanwhile,the expression of inducible nitric oxide synthase（iNOS）,neuronal nitric oxide synthase（nNOS） and endothelial nitric oxide synthase（eNOS） were detected by immunohistochemistry. Results The movement of contents from the proximal to the distal colon and rectum in model rats was significantly slower than that in normal rats.In model rats,the efflux time of the first melena was（686±57） min,significantly longer than that in normal rats（608±46） min（P0.05）.The expression of iNOS in model group was significantly higher than that in control group（P0.05）.The expression of eNOS and nNOS were not significantly different between model group and control group（P0.05）. Conclusion The abnormal expression of iNOS in the colon of STC model rats suggests that iNOS may play an important role in the pathogenesis of slow transit constipation.
出处
《胃肠病学和肝病学杂志》
CAS
2011年第1期64-66,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
广西壮族自治区卫生厅自筹基金资助项目(Z2006016)
关键词
一氧化氮合酶
慢传输型便秘
Nitric oxide synthase
Slow transit constipationistry
