摘要
经不同农药处理４ ｈ 时，水稻联合固氮菌菌株ＮＧＨ１１１ 和ＥＨ１１１３ 存活率在４８．１％～５９．４％ 范围内，活菌的浓度大于１０９／ｍ Ｌ。用８ 种除草剂处理联合固氮工程菌（简称工程菌菌剂），存活率分别为３０．２％ ～８３．７％ 。在液体菌剂中加４％ 甘油，于室温条件下保存２ 个月，存活率为１０％ ，活菌数达１０８／ｍ Ｌ。固体菌剂以草炭为介质，先于２８℃培养４～５ ｄ，后置４℃保存３ 个月，存活率为５０％ ，活菌数达１０９／ｇ。
The viabilities ofstrains NGH111 and EH1113 treated for 4 hours by several different pesticides w ere betw een 48.1% ～59.4% ,and the living bacteria w ere m ore than 109/m L.During the treatm ents ofvarious herbicides for 4 hours,the viabilities falldow n to 30.2% ～83.7% .Afterthe liquid cellculture containing 4% glycerolhad been stored atroom tem perature for tw o m onths,the viability ofthe bacteria w as 10% and the num ber of living bacteria w as 108/m L.When cells w ere innoculated into peat and cultured at 28℃for 4～5 days and then the solid cellculture w as preserved at 4℃for 3 m onths,the viability ofcells w as 50% and the num berofliving cells w as 109/g for the culture.
出处
《上海农业学报》
CSCD
1999年第4期64-66,共3页
Acta Agriculturae Shanghai
基金
国家高技术发展计划资助
上海市科技发展基金
关键词
固氮菌
农药
菌剂保存
水稻
存活率
Nitrogen fixing bacteria
Pesticide
Herbicide
Cellculture preservation
