摘要
采用热抽提和ＴｒｉｔｏｎＸ１００ 处理２ 种方法提取大肠杆菌ＥＬ１ 株和肺炎克雷伯氏杆菌ＥＬ２ 株表面抗原，经胃蛋白酶处理、ＳＤＳＰＡＧＥ 及免疫印迹分析表明，ＥＬ１ 、ＥＬ２ 株主要表面抗原耐热，对胃蛋白酶不敏感，分子量分别是２０ＫＤ、３３ＫＤ 和３６ＫＤ、４３ＫＤ，其抗原成分是多糖或多糖磷脂和蛋白质的复合物。二菌株表面抗原具有各自菌种的特征，可作为菌种分类鉴定的重要指标。此外，热抽提法提取细菌表面抗原的效果优于Ｔｒｉｔｏｎ Ｘ１００。
The surface antigens of E coli(Strain EL 1) and KL Pneumoniae (Strain EL 2)were extracted by heat treatment or by triton X 100 treatment and were treated with proteiase k The antigens,after analysis by polyacryamide gel electrophoresis and immunoblotting,have been shown to be resistant to heat and nonsensitive to proteiase k The molecular weights of the antigens were 20KD、33KD and 36KD、43KD respectively They were composed of polysaccharid or the complex of polysaccharid phosphatied and protein Because the surface antigens in two strains were not common,they had the species character and could be considered a key index in classified identification of species Moreover,the effect of heat extraction in extracted resistance heat surface antigen of bacteria was better than Triton X 100
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1999年第6期558-561,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
四川省教委重点科研资助
