摘要
本试验应用聚丙烯酰胺垂直板电泳分离、分析了旋毛虫抗原的组分。电泳后经考马斯亮蓝染色的旋毛虫幼虫抗原呈现２０ 条蛋白质色带；经Ｓｃｈｉｆｆ 试剂染色呈现６ 条红色的糖蛋白色带。将电泳后的凝胶均匀分割Ａｇ１ 、Ａｇ２ 、Ａｇ３ 、和Ａｇ４４ 个组分。分别测定其蛋白质浓度为１２３７μｇ、２２５４μｇ 、２６３６μｇ、２６０４μｇ 。糖蛋白区带成分属于Ａｇ１ 和Ａｇ４ 。经ＥＬＩＳＡ 和免疫电泳检测，Ａｇ１ 组分的抗原活性最强，其次Ａｇ２ ，Ａｇ３ 抗原活性微弱，Ａｇ４ 无抗原活性。通过试验证实Ａｇ１ 组分具有最强的抗原活性，敏感度高，而且属于大分子糖蛋白成分，它是作为检测旋毛虫病的首选最佳抗原成分。
This test used PAGE to analyse the antigene components of the muscle larvae of Trichinella Spiralis After electrophoresis and staining with Coomassie Brilliant Blue G250,the antigene components of the T Spiralis were found to be composed of 20 protein bands By using the glycoprotein specific Schiff′s reagent,six of them were shown to be red glycoprotein band Then the gel was cut evenly into 4 parts,Ag 1,Ag 2,Ag 3,and Ag 4 The protein concentration of them were 12 37μg,22 54μg,26 36μg,and 26 04μg respectively The glycoprotein bands belonged to Ag 1 and Ag 4 separately Tested by ELISA and immunoelectrophoresis,Ag 1 was found to have the greatest activity,Ag 2 was the second,and the antigenicity of Ag 3 was quite weak,yet Ag 4 was shown to have no activity at all As a glycoprotein component,Ag 1 was proved to have the greatest activity and sensitivity,thus it′s the best choice for the testing of T Spiralis
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1999年第6期554-557,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
