摘要
１９９７ 年１ 月～１９９８ 年３ 月，从北京市２０ 个商品鸭场自然病死的北京白鸭和河南省与上海市部分鸭场的樱桃谷鸭分离到２７６ 株鸭疫里氏杆菌，采用凝集试验和琼脂扩散沉淀试验，对其进行了血清型的研究。其中７０ 株细菌为１ 型，６４ 株为２ 型，其余１４２ 株不与１ 、２ 型参考菌株的抗血清发生反应。进一步的研究将未能定型的１４２ 株细菌划分为４ 类：第１ 类有７８ 株（ 暂称为ＣＡＵ３型） ，第２ 类有６ 株（ＣＡＵ４ 型） ，第３ 类有５２ 株（ＣＡＵ５ 型） ，剩余６ 株为第４ 类，不能与１ 、２ 、ＣＡＵ３ 、ＣＡＵ４ 、ＣＡＵ５ 型参考菌株的抗血清发生反应，这６ 株细菌和ＣＡＵ３、ＣＡＵ４、ＣＡＵ５ 三种血清型的参考菌株均不与８ 个ＮＡＤＣ的血清型即１ 、２ 、３ 、５ 、６ 、７ 、８ 、９ 型的高免血清形成可见的沉淀线。结果说明我国北京地区的２６０ 株鸭疫里氏杆菌至少属于６ 种血清型，而来自河南省的１０ 株细菌属两种血清型，来自上海地区的６ 株细菌至少属于４ 种血清型。１ 、２ 、ＣＡＵ３ 、ＣＡＵ５ 四种血清型占总分离株的９６ ％ ，是目前我国北京地区肉鸭场主要流行的血清型。
Two hundred and seventy six strains of Riemerella anatipestifer(RA) isolates from commercially raised,clinically diseased ducks in China were serotyped by agglutination and agar gel precipitin tests.Antigens from 70 field isolates reacted with the antisera to serotype 1,antigens from 64 isolates reacted with the antisera to serotype 2,antigens from the remaining 142 isolates did not react and were not serotyped with available antisera to NADC serotypes 1,2,3,5,6,7,8,9.Additional investigations showed that the remaining 142 isolates represented at least 3 serotypes which were named tentatively as serotype CAU3,CAU4 and CAU5.78 isolates belonged to serotype CAU3,6 isolates belonged to serotype CAU4,52 isolates belonged to CAU5 and 6 isolates were not react with antisera to any of these serotypes.The results indicated that 276 R.anatipestifer isolates from ducks in China during 1997 1998 fell into at least six serotypes.Serotype 1,2,CAU3 and CAU5 constituted a majority of the isolates(96%).
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1999年第6期536-542,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
