摘要
目的：建立毛细管电泳法测定麻杏石甘汤中钙镁含量的方法,并测定不同配伍麻杏石甘汤中钙镁的溶出量。方法：采用L9（34）正交设计,用毛细管电泳法测定钙镁的含量。电泳条件：背景缓冲液为6.4 mmol.L-1咪唑溶液（冰乙酸调pH至3.5）;检测波长214 nm;分离电压10 kV;重力进样（8 cm×7 s）;柱温室温。结果：钙离子测定的线性范围0.009 689.68mg.L-1,相关系数0.998 1,检测限0.002 1 mg.L-1,定量限0.007 1 mg.L-1,平均回收率100.4%;镁离子测定的线性范围为0.0110 mg.L-1,相关系数为0.995 9,检测限为0.002 8 mg.L-1,定量限0.008 9 mg.L-1,平均回收率96.4%。结论：本法快速简便、灵敏度高,可作为麻杏石甘汤中钙镁含量的测定方法。麻杏石甘汤中麻黄和杏仁对钙的溶出量影响显著,炙甘草对钙的溶出量影响不显著;麻黄对镁的溶出量影响显著,而杏仁和炙甘草对镁的溶出量影响不显著。
Objective： To establish a method for the determination of gypsum and magnesium in decoction by capillary zone electrophoresis and study the influence of different compatibility on the contents of calcium and magnesium dissolution.Method： Nine decoctions with gypsum were prepared and analysed by L9（34）orthogonal design and the contents of calcium and magnesium ions were determined by the set method.The conditions of the experiment were a running buffer of 6.4 mmol·L-1 imidazole solution（glacial acetic acid ajusted pH to 3.50）and an applied voltage of 10 kV（room temperature）.Samples were introduced by hydrodynamic injection（8 cm×7 s）and determined with on-column UV monitoring at 214 nm.Copper sulphate was chosen as the internal standard.Result： The linear responses covered the range from 0.009 68 mg·L-1 to 9.68 mg·L-1（r=0.998 1）for calcium,the detection limits（S/N=3） and the quantitation limits（S/N=10）of clalcium were shown to be 0.002 1 mg·L-1 and 0.007 1 mg·L-1,respectively.The average recovery for calcium was 100.4%.The linear response covered the range from 0.01 mg·L-1 to 10 mg·L-1（r=0.995 9）for magnesium.The detection limits（S/N=3）and the quantitation limits（S/N=10）of magnesium showed to be 0.002 8 mg·L-1 and 0.008 9 mg·L-1,respectively.The average recovery for magnesium was 96.4%.Conclusion： The method is simple,rapid,cost-effective and precise with satisfactory results.The influence of Ephedra and Semen Armeniacae Amarum on the contents of gypsum dissolution are significant,while that of Radix Glycyrrhizae on the contents of gypsum dissolution is insignificant.The influence of Ephedra on the contents of magnesium dissolution are significant,while that of Semen Armeniacae Amarum and Radix Glycyrrhizae on the contents of magnesium dissolution are insignificant.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第22期2985-2989,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(20905019
21011140338)
河北省自然科学基金(B2010000209)
河北大学自然科学基金(2007-111)
关键词
麻杏石甘汤
毛细管电泳法
钙
镁
间接检测
配伍
Maxing Shigan decoction； capillary electrophoresis； calcium； magnesium； indirect detection； compatibility
