摘要
根据miRNA序列在植物中的高度保守性,通过拟南芥、水稻、玉米等植物中已知的miRNA序列与马铃薯的表达序列标签(EST)、基因组测序序列(GSS)以及核酸数据库中的序列进行比对,最后通过在线比对软件blastx去掉编码序列,共发现32条新的miRNAs,并且这32条前体均可折叠形成miRNA家族的标准二级结构;利用新发现的马铃薯miRNAs通过在线软件miRU和miRU2与编码蛋白的数据库比对,预测到了100个靶基因,分别编码与马铃薯生长发育、新陈代谢、信号转导、转录调节、胁迫反应等相关的蛋白.
According to the conservative property of miRNAs in plant,a total of 32 novel miRNAs were identified from potato following a range of filtering criteria by using previously deposited miRNA sequences from Arabidopsis thaliana,Oryza sativa and other plant species to blast potato expressed sequence tags （EST）,genomic survey sequence （GSS） and nucleotide databases,and removing the protein coding genes by on line software blastx.All the potential miRNA precursors could be folded into the typical secondary structure of miRNA family.Using these miRNAs sequences,100 target genes were identified by blasting the databases of cording protein using on line software miRU and miRU2.These genes encode protein involved in growth and development,metabolism,signal transduction,transcriptional regulation and stress responses.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2010年第6期43-48,65,共7页
Journal of Gansu Agricultural University
基金
国家自然科学基金项目(30871573)
国家"863"项目(2009AA10Z103)
