摘要
构建编码ＨＢｓＡｇ 蛋白的重组真核表达质粒ｐＣＲ３－１Ｓ作为ＨＢＶ 基因疫苗， 免疫接种Ｂａ１ｂ／ｃ 小鼠。以重组质粒ｐＣＲ３－１Ｓ转染的Ｓｐ２／０ 细胞作为靶细胞， 采用５１Ｃｒ ４ｈ 释放法， 体外检测免疫小鼠的淋巴细胞杀伤功能。结果显示， 与空载体对照组相比较， ＨＢＶ基因疫苗可诱导Ｂａｌｂ／ｃ 小鼠产生ＨＢＶ 特异性细胞毒性Ｔ 细胞应答（ Ｐ＞ ０－０５） ， 提示以Ｓｐ２／０ 基因转染的细胞作为靶细胞， 检测免疫Ｂａｌｂ／ｃ 小鼠淋巴细胞的杀伤功能是可靠的。
Recombinant eukaryotic expression plasmid pCR3 1 S encoding S protein of HBV was constructed as HBV genevaccine Sp2/0 cells were transfected with recombinant plasmid pCR3 1 S as target cells Then cellular immune response of Balb/c mice inoculated with the vaccine against HBV was detected by 4 hour 51 Cr release assay The results showed that: compared with plasmid vector pCR3 1, the vaccine could strongly induce cytotoxic activity of lymphocytes to HBV in immunized mice(P<0 05) It was possible that Sp2/0 cells transfected with genes were used as target cells to test cytotoxic activity of lymphocytes from immunized Balb/c mice
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1999年第4期285-287,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金!资助项目
No-39770665
